Abstract Book - 3rd International Symposium on Medicinal Plants ...

species. With regard to the results of the antioxidant capacity and cytotoxic effect it follows the
need to assess more biological activities and to identify the metabolites of interest in each species.
Also it should be noted that it has been revealed the importance of the use of an emerging
technique as CE-MS in metabolomic studies.
Key words: Comparative study, metabolomic profile, taraxacum
3.39 Extraction of Antioxidants in Oil Palm Fruit Sterilization Derived
Condensate
Nipon Pisutpaisal 1,2 and Tipakorn Suwannarat 1
1 Division of environmental technology, Department of Agro-Industrial Technology, Faculty of
Applied Science, King Mongkut’s University of Technology North Bangkok, 2 The Research and
Technolo Center for Renewable Products and Energy, King Mongkut’s University of Technology
North Bangko. Thailand.
<strong>Abstract</strong>: This research harvested the solid power in oil palm fruit sterilization derived
condensate using electrocoagulation before the antioxidants in the powder were extracted. The
optimal conditions for the electrocoagulation were 1.3 ampere and 60 minute retention time. At
this conditions, chemical oxygen demand (COD) in the condensate was removed approximately,
74.1%. Methanol is the best solvent to solubilize antioxidants from the powder. DPPH (2,2-
diphenyl-1-picrylhydracyl radical, DPPH) test confirmed that methanol extract contained
antioxidants. The binding percentage of the methanol extract with free radicals was 68.8%. The
antioxidants in the methanol extract were isolated and purified by using Liquid Chromatography
and Thin layer chromatography. The purified antioxidant is anisole and esters of the omega -3
(Omega-3) which is connected to the ethylene glycol after chemical identification using Fourier
trans form infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NRM)
were performed.
Key words: Antioxidants, electrocoagulation, methanol, oil palm, solid power.
3.40 Phytochemical Study of Ethyl Acetate Extract and Antioxidant Activity
of the Species Genista quadriflora (Fabaceaes)
O. BOUMAZA 1 ; R. BOUKAABECHE 1 ; R. MEKKIOU 2 ; R. SEGHIRI 2 ;
D. ZAMA 1 ; D. SARRI; F. BENAYACHE 2 ; and S. BENAYACHE 1 .
1 Laboratory of Natural Resource Development, Department of Chemistry, Faculty of Exact
Sciences, University Mentouri Constantine . . 2 Laboratory of phytochemical analysis Physico-
Chemical and Biological Chemistry Department, Faculty of Exact Sciences, University Mentouri
Constantine.
<strong>Abstract</strong>: Genista species contain a variety of secondary metabolites of various types,
especially isoflavonoids, which have been shown to be biologically active. In a continuous of our
studies of Algerian medicinal and endemic plants of the Genista species (Fabaceae). Aerial parts
of Genista quadriflora were dried (1130 g), and macerated with MeOH–H2O (80:20, v/v) for 24
hours three times. The crude extract was concentrated at room temp. and diluted with 500 ml
H2O. The remaining aqueous solution was extracted successively with petroleum ether, CHCl3,
EtOAc and n-BuOH. The organic layers were dried with Na2SO4 giving after removal of solvents
under red. Pressure, petroleum ether (0.25g), CHCl3 (3g), EtOAc (6g) and n-BuOH (60g)
extracts respectively.The ethyl acetate extract was chromatographed on silica gel by gradient
elution with CHCl3-MeOH, the isolation and purification was performed by TLC on silica gel,
leading to some compounds (flavonoids and isoflavonoids).These compounds were identified by
spectral analysis.The extracts of G. quadriflora were examined for in vitro antioxidant properties
using DPPH test. The results showed that a different extracts had significant activity, On the basis
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