Abstract Book - 3rd International Symposium on Medicinal Plants ...

5.11 Antioxidant and Antifeedant Activities of Origanum glandulosum Desf.
Acetone Extract
Belhattab Rachid 1 and Azucena Gonzalez- Coloma 2
1 Biochemistry Department Faculty of Nature and Life Sciences, University Ferhat Abbas- Setif
19000, Setif, Algeria. 2 ICA, CSIC, 28006, Madrid, Spain.
<strong>Abstract</strong>: Origanum glandulosum Desf .an endemic species growing wild in Algeria belongs to
the Lamiaceae family. The plant is largely used as a beverage and also as source of medicines to
cure several pains such as rheumatism, cough and digestive disorders. Acetone extract of the
crushed aerial parts of the plant was obtained using a Soxhlet apparatus. Total phenolic content
determined spectrophotometrically according to Folin-Ciocalteu method in terms of cafeic acid
equivalent was 100,6 mg/100ml extract (38.7%w/w). The radical scavenging activity evaluated by
the DPPH test afforded 76.0% when compared to cafeic acid (100%), whereas the antifeedant
assay was assessed on three insects Spodoptora littoralis Boisd. (Lep:Noctuidae), Myzus persicae
and Ropalosiphum podi fifth instar larvae. The amounts of plant consumed have been recorded
for S.littoraalis as well as the number of insects (Myzus persicae and Ropalosiphum podi) settled
on each leaf disk.
Key words: Acetone extract, antifeedant, antifungal, insects, Origanum glandulosum.
5.12 Effects of Wild Rose (Rosa sp.) Crude Seed Extracts on Legume Aphids
Ben Cheikh-Affene Zohra 1 , Chaieb Ikbal 2 , Haouala Faouzi 3 and Harzallah-
Skhiri Fethia 4
1 Department of Horticultural Science and Landscape, Higher Agronomic Institute, University of
Sousse. 2 Laboratory of Entomology, Regional Centre of Recherche in Horticultural and Organic
Agriculture. 3 Department of Agronomy and Plant Biotechnology, National Institute of Agronomy of
Tunisia, University of Carthage. 4 Laboratory of Genetic, Biodiversity and Valorisation of
Bioresources, Higher Institute of Biotechnology, University of Monastir. Tunisia.
<strong>Abstract</strong>: Crude hexane seed extracts of four wild species of roses of Caninae Section: Rosa
canina L., Rosa dumetorum Thuiller., Rosa pomifera Herm. and Rosa rubiginosa L. were screened
for their insecticidal activity on two aphid species: black bean aphid (Aphis fabae Scop.) and pea
aphid (Acyrthosiphon pisum Scop.), feed on several cultivated species. Tests were conducted
using in vitro spraying of each rose seed extract at four doses: 0, 10, 20 and 40 mg ml -1 . Results
showed a bigger sensitivity of Aphis fabae to rose extracts. Indeed, at the dosis of 40 mg ml -1 , the
mortality varied from 25 to 47% for Aphis fabae and from 22 to 34% for Acyrthosiphon pisum. The
maximum of mortality for the two aphid species was observed with hexane extracts of R.
Dumetorum. This insecticidal activity may be due to the presence of unsaturated fatty acids in the
tested extracts.
Key words: Rosa canina, Rosa dumetorum, Rosa pomifera, Rosa rubiginosa, seeds, Aphis fabae.
5.13 The Insecticidal Effect of Some Medicinal Plants Extracts From the
South-West of Algeria
Boulenouar Noureddine 1,2 , Cheriti Abdelkrim 1 and Belboukhari Nasser 2
1 Phytochemistry & Organic Synthesis Laboratory, Bechar University. 2 Laboratory of Bioactive
Molecules and Chiral Separation, Bechar University, Bechar 08000, Algeria.
<strong>Abstract</strong>: Blatella germanica is one of the insect species that cause lot of problems for the
society. The existing treatments for this species are chemicals known as harmful for the
environment. In addition, this species is known for its resistance to many products. The purpose of
our study is the use of medicinal plants as source of bioinsecticide. In this study, the insecticidal
effect of extracts from four medicinal and/or poisonous plants from the South-West of Algeria:
Nerium oleander, Limoniastrum feei, Fredolia aretioides and Calotropis procera (two parts from
each plants) was evaluated against Blattella germanica. Aqueous extracts were obtained by
extraction with heat reflux procedure. Two supports were used to carry out the tests (nutritive
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