Abstract Book - 3rd International Symposium on Medicinal Plants ...

1.79 Antioxidant Activity of the Aqueous and Methanolic Extracts from
Peganum harmala Seeds
Saliha Dahamna 1 , Abir Rezzagui 1 ,Mounira Merghem 1 , Dalila Bouamra 1
,Daoud Harzalah 2 , Assia Belguet 1 , Soulef Boussahel 1 , Karima Yaici 1 ,and
Khadidja Dehimi 1
1 Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and
Physiology, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif . 2 Laboratory of
Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences,
University FerhatAbbas, Sétif, 19000, Algeria.
<strong>Abstract</strong>: Peganum harmala L. (Zygophyllaceae), commonly known as “Harmel” in North Africa,
is widely used in traditional remedies for the treatment of a variety of human ailments. The seeds
of this plant are reported to possess antidepressant, antidiabetic, antimicrobial, anticancerous and
hallucinogenic effect. In this work the antioxidant potential of the aqueous (AqE) and methanolic
(MeE) extracts of Peganum harmala seeds was investigated, and the second was the DPPH
radical scavenging activity. The analysis of the extracts showed that AqE contains the highest
amount of polyphenols followed by MeE (158,25 ± 6,80mg EAG /g dry extract and 98,44 ± 3,58mg
EAG /g dry extract, respectively) and the inverse in the flavonoids amounts with 7.68 ± 0.66mg EQ
/g dry AqE and 10.15 ± 0,017mg EQ /g dry MeE. The β-carotene/linoleic acid bleaching assay
revealed that the two extracts have an important antioxidant activity with 66.50 ± 1,46% of
inhibition for AqE and 79.71 ± 0,34% for MeE. High DPPH radical scavenging was observed in
MeE (0.194 ± 0.013mg/ml) followed by AqE (1.345 ± 0.036mg/ml). All together, these results
showed an antioxidant potential in both AqE and MeE, and suggested that these natural extracts
could be used to treat several diseases, especially, the stress oxidative-related disorders, through
the free radical scavenging activity and/ or the inhibition of lipid peroxidation.
Key words: Antioxidant activity, Free-radical scavenging activity, Peganum harmala L., DPPH.
1.80 Antioxidant and Anti-inflammatory Activities of Extracts Aqueous and
Ethanolic for Ginger.
SELLAL, A 1 ., SENATOR A 2 ., and Belattar R 3
1 Laboratory of Biochemistry, Faculty of Natural Sciences and Life, Department of Biochemistry,
University Ferhat Abbas Setif. 2 Laboratory of Biochemistry, Faculty of Natural Sciences and Life,
Department of Biochemistry, University Ferhat Abbas Setif. 3 Department of Ecology and Plant
Biology University of Constantine. Algeria
<strong>Abstract</strong>: In the present study, the activities of ethanolic and aqueous extracts from ginger
rhizome were evaluated firstly in vivo using acetic acid induced vascular permeability and xylene
induced ear edema by topical application in mice as a model of acute inflammation. The oral
administration of this extracts at dose of 400mg/kg, one hour before the induction of inflammation,
showed a high significant (p≤0,01) anti-inflammatory effect, compared to control group considered
as 100% of inflammation. Secondly, the antioxidant effect of ethanolic extract was studied only in
vitro (aqueous extract were studied in the priors works in our laboratory) using DPPH, total
antioxidant activity, metal chelating and s-carotene/linoleic acid tests. Results obtained showed
that this extract have a good scavenger effect towards DPPH radical and lipid peroxydation in total
antioxidant activity and s-carotene/ linoleic acid tests. In Contrast, this extract hasn‟t metal
chelating effect.
Key words: Inflammation, oxidation, free radicals, ginger.
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