Abstract Book - 3rd International Symposium on Medicinal Plants ...

mortality increased from 39.66 % at 0.125 mg /L to 91.77 % at concentration 1mg /L of
azadirachtin in direct effect. The median lethal concentration (LC 50 ) values was 0, 20 mg / L.
Cumulate mortality increased from 46.43% to 94.32% at 0,125mg/L and 1mg/L respectively. The
LC50 value for the indirect effect was 0.15 mg/L. Treatment resulted in a significant larvicidal effect
and an inhibition of adult emergence. Moreover, treatment with azadirachtin caused different types
of deformation in the larva, pupa and adult stages of mosquito.
Key words: Azadirachta indica, Culex pipiens pipiens, larvicidal, mortality.
5.3 In vitro Insecticidal Effect of Rosemary (Rosmarinus officinalis) Against
the Tomato leaf Miner (Tuta absoluta , Meyrick, 1917)
AMMAD Faiza , BOUTECHENT R., AMADA Farid, and AOUES K.
1 Department of Agronomy, Saad Dahleb University, PB. 270, route de Soumaa Blida, Algeria.
<strong>Abstract</strong>: The objective of this work has focused on evaluating the insecticidal activity (in vitro)
of an essential oil of Rosmarinus officinalis against the larvae of Tuta absoluta. The hydro
distillation as a method of extracting the essential oil of the plant tested (Rosemary) and Gaz
Phase Chromography (GPC) as a method for separating the complex substance of volatile
products and the identification and quantification of chemical compounds, the insecticidal potential
of the essential oil was evaluated by adding to 4 different doses (D1 :1ml H E +99ml Tween
,D2 :1.5 ml H E +98.5ml Tween . ,D3 :2ml H E +98ml Tween ,D4 ; 2.5ml H E +97.5 ml Tween .
The application of treatment was on Tuta Larvae (L2et L3) by contact. The control larvae were
treated by contact only by Tween 80 (3%), the counting of dead larvae was performed 24 hours,
48 h, 72 h and 96 h after each treatment. Statistical Method was done using: SYSTAT,
ver.12.SPSS 2009 and GLM (General Linear Model) and calculation of lethal concentration that
produce 50% of mortality. The analytical study of essential oil by (GPC), showed the presence of
sixty (16) chemical compounds: Cineole (56.90%) followed respectively by the pinene and
camphor (16.75% and 10.17%),α cadinene ( 0.0311%) and others. The essential oil of rosemary
used introduced a gradation of insecticide activity against larvae of the tomato leaf-miner by dose
and exposure time. The Lethal concentration (LC50) that induces 50% mortality of the study
population is 1.6mg/cm3. It is concluded that the essential oil of rosemary has toxic effect by
contacting and that substances used have a strong insecticide effect.
Key words: In vitro, Rosemary, Tuta absoluta meyrick.
5.4 Effectiveness of Certain Chemical and Safe Alternative Compounds as a
Management Tool of the Cotton Seed Bug Oxycarenus hyalinipennis
(Costa) Infesting Okra
Amro, Mohamed A 1 . and Abd El-Rahim, Gamal H 2 .
1 Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt.
2 Horticulture Department, Faculty of Agriculture, El-Azhar University, Assiut, Egypt.
<strong>Abstract</strong>: Population fluctuations of the cotton seed bug Oxycarenus hyalinipennis (Costa) was
monitored on okra (Abelmoschus spp.) in Assiut governorate, northern Upper Egypt during 2009
and 2010 okra growing seasons. In both seasons the pest showed two peaks. The first peak was
recorded in the third week of July, while the second one was recorded in mid August. Gradually
increase of the pest numbers was detected before each peak and coincided with the increase of
the nymphal stage populations. This could be considered as a prove that O. hyalinipennis may
have two generations on okra planted in northern Upper Egypt. The ability of certain chemical and
safe alternative compounds in reducing O. hyalinipennis populations in okra dry fruits was
determined. The chemical compound Sumithion 50% EC reduces the pest numbers by 92.21%.
However, the tested safe alternative compounds had less effectiveness (ranged 37.58% to
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