Abstract Book - 3rd International Symposium on Medicinal Plants ...

1.56 Acute and Chronic Toxicity of Aqueous Extract of Ruta montana L. in
Rodents
Merghem Mounira 1 , Dahamna S 1 ., Rezzagui A 1 ., Boussahel S 1 ., Belguet A 1 .,
Dehimi K. and Harzallah D. 2
1 Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and
Physiology, Faculty of Natural and Life Sciences, University Ferhat Abbas. 2 Laboratory of Applied
Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat
Abbas, Sétif, 19000, Algeria.
<strong>Abstract</strong>: The objective of this study was to evaluate the acute and chronic toxicity of aqueous
extract of Ruta montana L. in mice and rats. For the acute study, aqueous extract of Ruta Montana
L. was administered to mice in single doses of 0-12 g/kg given by gavage. General behavior,
adverse effects and mortality were determined for up to 14 days. In the chronic study, the extract
was administered orally at doses of 0, 100, 300 and 600 mg/kg daily for 90 days to rats.
Biochemical and hematological parameters were determined after 30 and 90 days. Enzyme
activities were assayed in the plasma samples obtained. AST (GOT), ALT (GPT), Cholesterol,
Triglyceride and Glucose. The results showed a decrease in RBC; WBC after one month (chronic
dose) of treatment (in males 75.48% &51.47% respectively), however an increase was
unregistered after 3 months (in males 130% & 171% respectively). This probably explained by the
effect of this plant extract on the erythropoiesis. A decline was observed on plasma enzyme
activities in both GOT& GPT on males after one month by (51.67% & 68.6 % respectively). On the
other hand, an increase after 3 months (acute dose) was recorded (on males 81.67% in females
184.61% respectively). These explain a high energy-generating product. A decrease was found in
cholesterol and triglyceride by 95.55% & 78.36% in the plasma enzyme activity. In acute toxicity,
there was no mortality and signs of toxicity. In Chronic toxicity, no mortality was observed,
biochemical and hematological analysis showed no significant changes in any of the parameters
examined of both sexes. Urine analysis was negative for glucose, bilirubin, ketones, blood, protein,
nitrite and leukocytes in the control and treatment groups. There were no significant differences in
the body and organ weights between controls and treated animals of both sexes. The
histopathological studies have been carried out (results not shown).
Key words: Acute and chronic toxicity, aqueous extract, medicinal plants, Ruta montana L.
1.57 Studies of Anti Bacterial and Antioxidant Potential of Methanol Extract
/Fractions of Peganum harmala
Meriem DJARMOUNI 1 , Abderrahmane B 1 ., Naouel B., Hayet T 1 ., Sabah B 2 .,
Farida B., Ameni D., Fatima Z. and Lekhmici A 1 .
1 Department of biochemistry Faculty of Biology, University Ferhat Abbas of Setif.. 2 Department of
biochemistry Faculty of Biology, University Brdj Bou-Arréridj.
<strong>Abstract</strong>: Peganum harmala L. seeds (Pgh) were extracted with solvent of varying polarity
allowed their separation into crude extract (CE), chloroform extract (CHE), ethyl acetate extract
(EAE) and aqueous extract (AqE). The highest total polyphenols and flavonoids amounts were
recorded in CHE (66.29 ± 1.57 mg EGA / g dry extract and 13.88 ± 0.13 mg EQ / g dry extract),
followed by EAE (58.1 ± 1.57 mg EGA / g dry extract and 12.18 ± 0.08 mg EQ / g dry extract,
respectively). The antioxidant activities of these extracts were estimated by superoxide scavenging
ability and reducing power using (PMS-NADH-NBT) and FRAP assay. The result showed that
CHE have the stronger superoxide scavenging effect with IC 50 0,395± 4,9117E-05 mg/ml, followed
by EAE and CE with IC 50 of 1,644± 0,023 and 3,33± 0.047 mg/ml, respectively. In ferric reducing
power assay, the results showed that extracts exhibited a good reducing power, and the highest
power was recorded for CHE (TAP = 5,85±0,145 mM Fe +2 /g of extract). The antibacterial effect of
CE, CHE, EAE was assessed by the disc method. . The results showed that this plant has a very
important antibacterial effect on strains E. coli ATCC 25922, S. aureus ATCC 25923 and P.
aeroginosa ATCC 27853. The most sensitive strain was S. aureus to both EAE and CHE with a
30