Abstract Book - 3rd International Symposium on Medicinal Plants ...

non-serpentine, ехеpt of content of tannins in samples from H. cerastoides. It could be suggested
that these levels are in relation with the specific stress conditions of the habitat.
Key words: flavonoids, flowering season, habitat, Hypericum, Serpentine, tannins.
3.25 Analysis of Extracts of Different Polarity from Yarrow Herb
Kyslychenko O., Koshoviy O., and Komissarenko A.
The National University of Pharmacy, Kharkiv, Ukraine.
<strong>Abstract</strong>: Yarrow herb (Achillea millefolium) is widely used in official medicine as hemostatic,
antimicrobial and anti-inflammatory remedy. There are approximately 20 drugs at the market of Ukraine
and Russia Federation that contain biologically active substances (BAS) of yarrow herb. Nevertheless,
there is no mono-drug on yarrow basis that may be some kind of omission as this raw material has a
broad spectrum of pharmacological activity and can become a basis for the new antimicrobial and antiinflammatory
remedies. While making the literature review we found out that extraction parameters of this
plant material are not justified in a proper way. That is why the aim of our research was to find the
extractant that provides the best extraction of BAS from yarrow herb and to study the chemical content of
these BAS and their antimicrobial activity. Solvents with different dielectrical constants such as ethanol,
water, ethylacetate, hexane, chloroform, and acetone (1:10) were used to obtain extracts from yarrow
herb. 1% ethanol solutions were prepared from obtained extracts and were further used for the
analysis. Qualitative reactions, paper chromatography and TLC were used to study the qualitative
composition. Hydroxycinnamic acid derivatives, flavonoids, tannins, coumarins, terpenoids and
chlorophylls were detected in yarrow herb extracts. The quantitative content of main BAS groups was
determined. Quantitave determination of phenolic compounds (hydroxycinnamic acid derivatives,
flavonoids, and chlorophylls) was made by spectrophotometry according to the wavelength.
Hydroxycinnamic acid derivatives were determined in terms of chlorogenic acid (λ=327 nm), the total
flavonoid content in terms of rutin (λ=417 nm) after formation of complex with Aluminium chloride, the total
phenolic compound content in terms of gallic acid (λ=270 nm) and chlorophylls (λ=649, λ=665 nm). The
essential oil content was performed according to the State Pharmacopoeia of Ukraine by the
hydrodistillation technique in presence of water vapors. It was found out that ethanol in concentration from
70% to 96% as well as ethylacetate provided the best extraction of BAS. Studies of antibacterial activity
were conducted by diffusion to agar technique. Yarrow herb extracts show antimicrobial activity to S.
aureus, B. subtilis, S. pyogenosa and E. coli but has a very light impact on Proteus vulgaris, P.
aeruginosa, Candida albicans. Ethanol and ethylacetate extracts were the most active. To make a
conclusion, chemical content and antimicrobial activity of ethanol, water, ethylacetate, hexane,
chloroform, and acetone extracts from Achillea millefolium herb were studied. That will be a basis
for new drugs development.
Key words: Antimicrobial, essential oil, gallic acid, phenolic, Yarrow herb.
3.26 Flavonoids of Some Salvia Species wide-Spread in Ukraine
Kyslychenko, Viktoriia., Koshoviy, Oleg., and Komissarenko, Andriy
The National University of Pharmacy, Kharkiv, Ukraine
<strong>Abstract</strong>: Salvia genus consists of about 600 species, 30 of them are found in Ukraine. Officinal
raw material in Ukraine is folia S. officinalis. S.officinalis is native to Asia and in Ukraine it‟s
cultivated. Main therapeutic activities of the drugs, containing its extracts are antibacterial and antiinflammatory.
S.officinalis leaves are used in pharmaceutical industry as the source of essential oil
as well as are needed for tincture production, acetone extract "Salvin". S.sclarea essential oil
which possesses broad spectrum of antibacterial activity is also used. The analysis of literature
has shown that isoprenoids such as mono- and sesquiterpenoids, phenilpropanoids, di- and
triterpens, fatty acids were studied the best. There is an expediency of phenolic substances study,
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