Abstract Book - 3rd International Symposium on Medicinal Plants ...

1.26 Antioxidant and Antiulcerogenic Effects of Globularia alypum L.
Extracts
Derrafa I., Benchikh Fatima, Amira S., Khennouf S. and Dahamna S.
Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University
Ferhat Abbas, Setif, Algeria.
<strong>Abstract</strong>: Globularia alypum L. is widely used as a medicinal plant in Algeria. In this context, we
estimated the antioxidant and anti ulcerogenic activity of various solvent extracts prepared from
the aerial parts of this plant. The quantitative analysis of plant material reveal that these extracts
are rich in polyphenolic compounds (41.04 to 136.66 mg EAG/G of extract) for total polyphenols ,
(10 to 15.52 mg ER/G of extract and 5.04 to 6.68 mg EQ/g of extract) for flavonoids and (52.88 to
164.33 mg ETA / G of extract) for tannins. The evaluation of the scavenging capacity of the
extracts with respect to DPPH shows that the crude methanolic (CrME), the aqueous (AqF) and
the ethyl acetate (EaF) extracts were the most active, with an IC 50 of 33.32 , 36.12 and 38.29
µg/ml respectively. The inhibition of the coupled oxidation of the β-carotene / linoleic acid assay
showed an important antioxidant activity of 79.10 for CrME, 73.37 for AqF, and 70.17 for the
chloroformic extract (ChF). The ferrous ion chelating capacity test showed that the crude aqueous
(CrAE), AqF and CrME extracts were the most active. Oral administration of (100, 300 and 600
mg/kg) of Globularia alypum L. extracts reduced the severity of gastric lesions induced by 70%
ethanol in the mouse. These results show that this plant is a good source of antioxidant molecules
and may explain in part, its pharmacological properties and its traditional therapeutic uses.
Key words: Antioxidant activity, ethanol, gastric ulcer, Globularia alypum, polyphenols.
1.27 Bioinformatic Analysis and Study of the Antiulcerous of an Active
Principale Compared to Gastric Medecines
DJALILA BOUDEMAGH., 1 ZAWANI M. 2 , VIJAY M 3 ., and TAIBI BEN H. 4
1 Département de Génie des Procédés, Université Ferhat Abbas de SETIF, Algérie. 2 Ecole
Nationale Supérieure Vétérinaire d'Alger-16000, Algérie. 3 Department of Chemistry, Vidya Bharati
College, Amravati, India- 444 602. Laboratoire Chimie des Matériaux, Faculté Sciences, Oujda-
60000, Maroc.
<strong>Abstract</strong>: The anti-ulcer activity of active principle has been carried out in albino rats. First for all
study on small groups of animals was conducted to know the approximate ulcer protective activity
of the active principle compared to the different gastric medicines Maalox, Ranitidine and
Omeprazole. Anti-ulcer activity for product was studied in rats, in witch gastric ulcers induced by
oral administration. The reduction of ulcer index as well as gastric out put in product treated
animals was found to be statistically significant with respect to control animal. The average ulcer of
control was found to be varied from (1.4 ± 1.14) to (0.2 ± 0.447) for a dose of 250 mg/kg. Thus
results tend to confirm that the active principle has a preventive role in drug induced ulceration.
Key words: Anti-ulcer activity, gastric medicines, ulcer index.
1.28 The Antioxidant and Lipid Peroxidation Activities of Rubus idaeus L.
Extracts
Djamila AMENI 1 , Abderrahmane BAGHIANI 2 , Saliha DJIDEL 3 , Sabah
BOUMERFEG 2 , Seddik KHENNOUF 3 and Lekhmici ARRAR 2 .
1 Department of Biochemistry, Faculty of Nature and Life Sciences, University Abderrahmane Mira
Bejaia. 2 Laboratory of applied biochemistry, Department of biochemistry, Faculty of Nature and Life
Sciences, University Ferhat Abbes Setif,. 3 Laboratory of Phytotherapy applied to chronic diseases
, Department of biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbes Setif,
Algeria
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