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Abstract Book - 3rd International Symposium on Medicinal Plants ...

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support: wafer, n<strong>on</strong> nutritive support: sawdust). For the tests with nutritive support the<br />

c<strong>on</strong>centrated extract and four diluti<strong>on</strong> were evaluated (1/2, 1/4, 1/6, 1/8). For the n<strong>on</strong> nutritive<br />

support, a quantity of 1g for 4ml of each c<strong>on</strong>centrated extract was tested. A coupling between the<br />

extracts was carried out to evaluate the effect of synergy. The tests were realized <strong>on</strong> adult insects.<br />

C<strong>on</strong>cerning the first support, the results did not show any insecticidal effect. Some extracts<br />

especially from toxic plants showed death rates between 20 and 80% by using the sawdust. The<br />

coupling of the extracts did not increase the insecticidal effect. This study has dem<strong>on</strong>strated that<br />

the extracts of certain plants c<strong>on</strong>tain bioactive substances which can be used as source of bioinsecticide<br />

against Blattella germanica.<br />

Key words: Bio-insecticide, Blattella germanica, medicinal plants, pois<strong>on</strong>ous plants, sawdust.<br />

5.14 Digestive Tract Histological Disorder of L5 Locust Schistocerca<br />

gregaria (Orthoptera, Cyrtacanthacridinae) After Ingesti<strong>on</strong> of Peganum<br />

harmala L. (Zygophyllaceae)<br />

Bounaceur F., 1 Milat-Bissaad F.Z. , 2 Outtar F. 3 and Doumandji Mitiche-B. 3<br />

1 Faculty of Natural Sciences and Life, University Ibn Khaldoun. Tiaret . 2 Department of Biology,<br />

Faculty of Science, University of Boumerdes M'Hamed Bouguera. 1 Nati<strong>on</strong>al School of Agr<strong>on</strong>omy<br />

ENSA, Department of Agricultural and Forest Zoology ENASA, Algeria.<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>: The Peganum harmala L. is known for its traditi<strong>on</strong>al use, the seeds have l<strong>on</strong>g been<br />

used as macrotis, anthelmintics, antispasmodic and in some cases against rheumatism and<br />

asthma. In the present work, we tested the effect of this plant <strong>on</strong> the gut histology of the 5 th<br />

nymphal instar of the desert locust. Because, is insect since c<strong>on</strong>sumes this plant despite that it be<br />

toxic. As the result, there was significant histological disorder in the digestive tract of the treated<br />

insect compared to the c<strong>on</strong>trol. Such disorders were manifested mainly in the midgut. However<br />

there was no similar disorder observed in the foregut and hindgut of the treated insects. The<br />

present results revealed the effectiveness of the Peganum harmala against the desert locust in<br />

causing detrimental histological disorders in its mesenter<strong>on</strong>.<br />

Keywords: Peganum harmala, Schistocerca gregaria haemolymph.<br />

5.15 Preliminary Study of the Effects of Rootstocks and Viroids <strong>on</strong> the<br />

Essential Oil Compositi<strong>on</strong> of the Sweet Orange “The Maltaise Demi<br />

Sanguine”<br />

Chammem Nadia 1 , Najar Asma 2 , Sifaoui Ines 3 , Jribi Chokri 3 , and Ben<br />

Abderrabba Manef 3<br />

1 Institut Nati<strong>on</strong>al des Sciences Appliquées et de Technologie (INSAT), Centre urbain Nord. B.P.<br />

676, 1080 Tunis, Tunisie. 2 Laboratoire de protecti<strong>on</strong> des végétaux, Institut Nati<strong>on</strong>al de la<br />

Recherche Agr<strong>on</strong>omique de Tunisie, Rue Hedi Karray, 2049 Ariana, Tunisie. 3 Institut Préparatoire<br />

aux Etudes Scientifiques et Techniques (IPEST) B.P. 51, 2070 La Marsa, Tunisie.<br />

<str<strong>on</strong>g>Abstract</str<strong>on</strong>g>: Besides tolerance against diseases, the use of rootstocks in citrus crops may be<br />

enhanced by studying some other important aspects like extracti<strong>on</strong> and analysis of essential oils<br />

(E.O) that are more and more required as active biomolecules. The presence of E.O in the<br />

different tree organs may be influenced by factors affecting the plant status. In the present work,<br />

this influence has been studied in relati<strong>on</strong> with viroid infecti<strong>on</strong> and the root stock species in the<br />

case of Tunisian Maltaise cultivar that was grafted <strong>on</strong> Citrumelo swingle and Citrus macrophylla<br />

and inoculated with exocortis and cachexia viroids. The analysis of terpenic compositi<strong>on</strong> of those<br />

E.O in the fruit exocarps showed that (i) Maltaise/Citrumelo swingle associati<strong>on</strong> of trees infected<br />

by exocortis gave two folds less of terpinen-4-ol and terpineol than the c<strong>on</strong>trol. At the qualitative<br />

level, exocortis infecti<strong>on</strong> in this case induced biosynthesis of new molecules e.g. n-decanal, cis-<br />

115

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