Abstract Book - 3rd International Symposium on Medicinal Plants ...

1.88 Secondary Metabolites and Free-Radical Scavenging Activity of the
Endemic Species Stachys mialhesi de Noé.
Souheila LAGGOUNE 1 , Assia ZEGHIB 1 , Francisco LEON 2 , Ignacio BROUARD 2 ,
Jaime BERMEJO 2 , Claude-Alain CALLISTE 3 , Jean-luc DUROUX 3 , Ahmed
Kabouche 1 , and Zahia KABOUCHE 1*
1 Laboratory of Therapeutic Substances (LOST), Department of Chemistry, Faculty of Sciences,
University Mentouri - Constantine, Chaabet Ersas Campus, 25000 Constantine, Algeria. 2 Instituto
de Productos Naturales y Agrobiolog ı´a-C.S.I.C.-Instituto Universitario de Bio-Orga´nica ‘‘Antonio
Gonza´lez’’, Universidad de La Laguna, Av. Astrof ı´sico F. Sa´nchez 3, 38206 La Laguna,
Tenerife, Spain. 3 Laboratoire de Biophysique, UPRES EA-1085, Biomolécules et cibles cellulaires
tumorales Faculté´ de Pharmacie, 2 rue du Dr. Marcland, 87025 Limoges Cedex, France.
<strong>Abstract</strong>: One diterpenoid, horminone (1), two flavonoid glycosides, apigenin-7-O-(6''-E-pcoumaroyl)-β-D-glucopyranoside(2),isoscutellarein-7-O-(2''-O-6'''-O-acetyl-ı-D
allopyranosy l-ı-Dglucopyranoside
(3), were isolated from n-butanolic extract of the aerial parts of Stachys mialhesi
de Noe´ (BESM). Two sterols, stigmasterol (4) and β-sitosterol (5), two lignans, (+)-sesamin (6),
new for the genus Stachys and (±)-paulownin (7), new in the Lamiaceae family, reported for the
second time from a natural source, and one acetylated flavones glycoside isoscutellarein-7-O-(2''-
O-6'''-O-acetyl-ı-D-allopyranosyl-ı-D-glucopyranoside (3), were isolated from the roots of
Stachys mialhesi de Noé. Surprisingly, 3g of compound (3) are gathered from 5g of roots extract.
Their structures were established on the basis of physical and spectroscopic analysis, and by
comparison with the literature data. The free-radical-scavenging property of the BESM and the
compound (3) were evaluated by the use of the Electron Spin Resonance (ESR) method in order
to visualize the inhibition of the 2,2-diphenyl-1-1picrylhydrazyl (DPPH) free radical. Our results
showed that S. mialhesi de Noé might be a source of natural antioxidants. The performed test
confirmed that antioxidant potential of the plant, representing by an abundant flavonoid glycoside
(3) in its root extract with a fairly high IC 50 value (0.066 ± 0.002 (mg/ml)) to scavenge DPPH free
radical.
Key words: Free radical scavenging activity, lamiaceae, lignans, Stachys mialhesi de Noé.
1.89 Lipolysis Activity of Herbal Extracts and Nano Formulation for Cellulite
Treatment.
Sucontphunt Apirada, Ondee T., Chutoprapat R., Chansriniyom C. and
Nimmannit U.
National Nanotechnology Center (NANOTEC), National Science and Technology Development
Agency (NSTDA), Klongluang, Pathumthani 12120, Thailand.
<strong>Abstract</strong>: Obesity is characterized by increasing of the number or size of fat cells (adipocytes),
or a combination of both. Localization of adipose deposits and edema within the subcutaneous
tissue caused cellulite which been described as resembling an orange peel-like appearance.
Approximately 85% of postadolescent women have some degree of cellulite. Therefore, the
objective of this study is to find natural ingredients that promote lipolysis to reduce cellulite
occurrence for incorporation in nano-cosmetic product for cellulite treatment. Selected herbs were
extracted with ethanol and then the supernatant were evaporated until dryness. Adipocytes were
maintained in 96 well- plate at 37°C in a humidified 5% CO 2 atmosphere. Various concentrations
of herbal extracts were treated to adipocytes at 37°C for 3 h. Lipolysis activity was determined by
measurement of free glycerol occurred from lipolysis using free glycerol determination kit. Various
Thai herbs were investigated for their lipolysis activity. It was found that extracts of Allium sativum
and Piper nigrum significantly increased lipolysis activity of adipocytes. At the concentration of 10
µg/ml, Allium sativum extract exhibited the highest lipolysis activity (142%). Therefore it was
selected to be the active ingredient in nano-cosmetic product for cellulite treatment. Nanoniosomes
having the size in nano-meter range that could enhance the penetration of active
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