Abstract Book - 3rd International Symposium on Medicinal Plants ...

4.5 Formation of Hawthorn, Crataegus azarolus L. Plants from Multiplication
of Buds In Vitro
Al-Mallah, M.K. 1 and AL-Hadeedy, S.H. 2
1 Forestry Department, College of Agriculture and Forestry.
2 Biotechnology Department, College of
Education-Mosul, University of Mosul, Iraq.
<strong>Abstract</strong>: The results proved that buds vegetative multiplication that excited from Hawthorn
trees produced numerous shoots from single bud by using agar solidified MS medium
supplemented with 5.0 mg/L BA, (Benzyl adenine). It was possible to enhance the multiplication
rate by adding 0.5 mg/L of Indole Butyric Acid (IBA) to the medium with decreasing concentration
of BA to 3.0 mg / L. This medium stimulates the formation of callus that differentiated to new
shoots.
Key words: Crataegus azarolus, hawthorn, in vitro, multiplication.
4.6 Improving Horse Chestnut Multiplication Rates and Embryo Quality
Ćalić Dušica, Devrnja Nina, Milojević Jelena, Tubić Ljiljana, and Zdravković-
Korać Snežana
Institute for Biological Research “Siniša Stankoviı“, University of Belgrade, Despot Stefan Blvd
142, 11 000 Belgrade, Serbia.
<strong>Abstract</strong>: Some of the horse chestnut (Aesculus hippocastanum L.) androgenic embryos in
microspores and anther culture were abnormal. Addition of abscisic acid in media with horse
chestnut androgenic embryos has circumvented the abnormal morphology. The best results were
achieved on medium with the lowest abscisic acid concentration (0.01 mg l -1 ) in microspore
culture. The microspore culture proved to be a better model system for albino embryo reduction
than anther culture. Also, impact of abscisic acid on secondary somatic embryogenesis was
investigated with the aim of improving multiplication rates and somatic embryo quality. With
increasing ABA concentration decreased number of secondary somatic embryos of about eight
times. Number of secondary somatic embryos was tree times higher on hormone-free medium
than on medium with the lowest ABA concentration. Secondary somatic embryo production on
roots was ten times higher than on cotyledons in both type of culture. Overall, the impact of ABA
on induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos
of this species may be helpful for further synthesis secondary metabolites in vitro and their
applicable in the pharmaceutical industry.
Key words: Abscisic acid, embryo morphology, secondary somatic embryogenesis.
4.7 Introduction and Breeding of Wild Growing Medicinal Plants in CentraL-
Europe
Éva Zámboriné -Németh
Corvinus University of Budapest, Department of Medicinal and Aromatic Plants.
<strong>Abstract</strong>: Wild growing plants are widely used in Central Europe based on traditional
knowledge. For centuries, the demand has been supplied by collection from wild habitats.
However, in the recent 20 years, an accelerated tendency can be observed for introduction several
wild growing species into agriculture. It can be traced back to different reasons.The increased
demand for high and reliable quantity of raw material as basis of industrial processing can not be
covered from natural populations. This is a specifically important question in case of rare and
endangered species, where collection would contribute to their extinction. Protected species are
only allowed to harvest with special permissions, regulated differently from country to country. In
some countries, collection from the wide. The most important stimulating factor for introduction of
98