Abstract Book - 3rd International Symposium on Medicinal Plants ...

as 89,007±1,914 % and 59,639±3,824 % for the methanolic and aqueous extract respectively. In
both tests the methanolic extract was the most active. On the other hand, total phenolics
determination in the test solutions was carried out according to the spectrophotometric Prussian
blue assay and the determination of flavonoids was performed using the method of aluminum
trichloride, (AlCl 3 ) al the results indicated that the methanolic extract has higher total phenolics and
flavonoids being of: 33.655±2.503mgGAE/g.extract for the first method and 61.127±1.217mgEQ/g;
129.681±1.546 mg ER/g, for the second one. According to al these results, we conclude that there
is a clear relationship between chemical composition of R. alaternus and its antioxidant activities.
Key words: Antioxidant activities, chemical composition, extracts Rhamnus alaternus L.
3.13 Optimisation of Solvent Extraction of Antioxidant Compounds
(Phenolic Compounds) from Algerian Mint (Mentha spicata L.)
Brahmi Fatiha 1 , Madani Khodir 1 , Rahmani Tiziri, Bousbaa Karima, Oukmanou
Sonia 1 and Chibane Mohamed 1
1 Laboratoire de Biomathématique, Biochimie, Biophysique et Scientométrie, Faculté des Sciences
de la Nature et de la Vie, Université Abderrahmane Mira, Bejaia, Algérie.
<strong>Abstract</strong>: The mints are herbs rich in essential oils and phenolic compounds that appear to be
the cause of prevention of many diseases. This study first allowed to quantify the levels
of polyphenols and flavonoids from mint: Mentha spicata L. harvested in Bejaia (Algeria) using
pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50%; and secondly, to
evaluate their antioxidant activities by two methods (radical scavenging activity using DPPH and
total antioxidant activity by phosphomolybdate test). The protocol elaborated by Singleton
and Rossi (1965) was used to quantify total phenolics. Flavonoids were evaluated
using Bahorun et al. (1996) protocol. The method ported by Stankevičius and al.
(2010) was adopted to determine radical scavenging activity of different extracts while
this ported by Jayaprakasha et Patil (2007) to determine total antioxidant activity. In light
of our results, it appears that ethanol 50% gave a better extraction rate for the plant studied
(14.1%). On the other hand, the ethanol extract of 50% presented the highest content in
polyphenols (39.473 ± 1.81 mg EAG / g DM). Consequently, it manifests the highest radical
scavenging activity. Regarding flavonoids, methanol was the best extractor. Whereas, the total
antioxidant activity was attributed to the acetonic (0,230 ± 0,015 AU) and ethanolic
extracts (0,222 ± 0,016 AU) without significant difference. The difference in the antioxidant
capacity of different extracts was probably due to the difference of the nature of the compounds
extracted with different solvents.
Key words: Antioxidant activity, extraction, Mentha spicata, phenolic.
3.14 Morphological and Biochemical Characteristics of Pollen Grains and
Bee Corbicular Pollen Collected from Sunflower (Helianthus annuus L.)
Brindza Jan 1 , Synytsya Andriy 2 , Bíro Daniel 1 , Ostrovsky Radovan 1 ,
Shevtsova Tetiana 3 , and Toth Dezider 1
1 Institute of Biodiversity Conservation and Biosafety, Faculty of Agrobiology and Food Resources,
Slovak University of Agriculture (SUA) in Nitra, Slovak Republic, 2 Department of Carbohydrates
and Cereals, Institute of Chemical technology (ICT) Prague, Czech Republic, National Aviation
University, Institute of Ecological Safety, Kiev, Ukraine 3
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