Abstract Book - 3rd International Symposium on Medicinal Plants ...

(11.3%). The antioxidant activity of the essential oils was determined employing two different assays:
DPPH• free radical scavenging assay and Phosphomolybdenum assay. The results showed that the
essential oils have an excellent reducing activity but low anti-radical power, by comparison with
those of the antioxidants of reference. These results show clearly that the essential oil extract is a
good antioxidant, and represent an important potential as a natural antioxidant.
Key words: Antioxidant, chemical composition, DPPH assay, essential oil, Saccocalyx
satureioides.
1.68 In Vitro Vasomotor Effects of Capparis spinosa Aqueous Extracts
Nadia BENZIDANE 1 , Imane K 1 ., Abderrahmane B 1 ., Noureddine C 1 ., Seddik
K 2 ., N. XAVIER 3 , and Lekhmici A. 1
1 Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life
Science, University Ferhat Abbas, Setif, Algeria. 2 INSERM U698, Faculté de Médecine Xavier
Bichat, Paris, France.
<strong>Abstract</strong>: Capparis spinosa (Capparidaceae) dicotyledons from the class of spermaphytes, is
an arbustive, enduring and woody plant, typically Mediterranean, largely used in folk medicine in
the Mediterranean countries including Algeria. The aim of the present research is to assess the in
vitro vasomotor effects of aqueous extract of different parts of Capparis spinosa (roots, leaves,
stems, flowers, fruits and kernels). Rings of thoracic aorta and windpipe of rat Wistar were isolated
streamlined cut and suspended by means of bath of organs containing 10 ml of Krebs
physiological solution. The addition of Capparis spinosa extracts with different concentrations
during the stage of contraction led by the phenylephrin for the thoracic arteries and acetylcholine
for windpipes showed a light vasodilatation. Another protocol, by incubation 30 mn with extracts at
different concentrations proves to be so efficient. Several doses (0,1 mg/ml, 1 mg/ml et 10 mg/ml)
have been used. The dose of 10 mg/ml showed a significant vasodilator effect for fruits and
kernels, and vasoconstrictor effect for leaves. This study is preliminary and cannot give an idea
neither of the mode of action, nor of substance (s) responsible of the expressed effects.
Key words: Aortic ring, aqueous extract, Capparis spinosa, medicinal Plants, vasomotor effect.
1.69 Inhibition of Stromelysine-1 By Chicoric Acid Derivatives Isolated from
Algerian Propolis.
Narimane Seguen 1,3 , Abdulmagid Alabdul Magid 2 , Martine Decarme 1 , Salah
Rhouati 3 , and Mesbah Lahouel 4
Frank Antonicelli 1 , Catherine Lavaud 2 , William Hornebeck 1 1 Unité Medyc, Université de Reims
Champagne Ardennes, UMR 6237 CNRS, IFR 53 Biomolécules, UFR de Médecine, 51 rue
Cognacq Jay, 51095 Reims.cedex, France. 2 Laboratoire de Pharmacognosie, Université de Reims
Champagne Ardennes, Institut de Chimie Moléculaire de Reims, UMR 6229 CNRS, IFR
53Biomolécules, Bâtiment 18, BP 1039, 51687 Reims cedex 2, France; 3 Laboratoire des produits
naturels d’origine végétale et de synthèse organique, Université Mentouri, Département de
Chimie, Constantine 25000, Algérie. 4 Laboratoire de phytopharmacologie, Université de Jijel,
Département de biologie, Jijel 18000, Algérie.
<strong>Abstract</strong>: Repeated exposure of human skin to sunlight is considered as a major factor in the
premature aging of skin (photoaging). The UV-irradiation caused excessive generation of ROS
thereby resulting in an oxidative stress condition. It is suspected that ROS are capable of
enhancing matrix metalloproteinase activity. Thus the approaches contracting ROS production
may be useful for the prevention of photoaging and skin cancer. Polyphenols are known as potent
antioxidants. In addition, they can decrease the level of MMPs production and increase the level of
procollagen synthesis. In this study, our focus was on the effect of propolis polyphenols on
stromelysin-1 (MMP-3), this enzyme occupies a central position in collagenolytic and elastolytic
cascades. We screened extracts of a propolis sample with the aim to isolate compounds able to
selectively inhibit this enzyme. A butanolic extract (B3) of the investigated propolis sample was
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