Abstract Book - 3rd International Symposium on Medicinal Plants ...

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1.43 Polyphenols and Antioxidant Properties of Extracts from Mentha pulegium L. and Matricaria camomilla L. KHENNOUF Seddik 1 , DJIDEL Saliha 1 , BENCHIEKH Dalila 1 , DAHAMNA Saliha 1 , CHAREF Noureddine 2 , BAGHIANI Abderahmane 2 , HARZALLAH Daoud 3 and ARRAR Lekhmici 2 1 Laboratory of phytotherapy applied to chronic diseases. 2 Laboratory of applied biochemistry. 3 Laboratory of applied microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif, 19000, Algeria. <strong>Abstract</strong>: This study was carried out to determine the antioxidant activity of Mentha pulegium L and Matricaria chamomilla L extracts. The methanolic extract (MeE) of Mentha pulegium L gave the higher yield (14, 4%) of extraction. Whereas the aqueous extract (AqE) of Matricaria chamomilla L had the highest yield (18, 56%) of extraction. Moreover, the ethyl acetate extract of Mentha pulegium L contains high amount of total polyphenols; tannins and flavonoids (191,99µg gallic acid equivalent/g; 265,33µg tannic acid equivalent/g; 110,37µg quercetin equivalent/g and 151,11µg rutin equivalent/g) respectively. This extract possessed high antioxidant activity (IC 50 =0,017mg/ml) in DPPH assay while the chloroformic extract (ChE) of this plant was stronger in the β carotene/linoleic acid assay. On the other hand, the ChE of Matricaria chamomilla L contains the higher value of flavonoids (197,43µg quercetin equivalent/g; 273,03µg rutin equivalent/g) whereas total polyphenols were higher in MeE (299,14µg gallic acid equivalent/g) and ChE showed high tannin values (245,11µg tannic Acid equivalent/g). Matricaria chamomilla extracts exerted high DPPH scavenging activity and a moderate antioxidant action in the β carotene/linoleic acid assay. It was concluded that the therapeutic use and biological activities of these plants could be attributed to phenolic compounds and its free radicals scavenging and antioxidant properties Key words: Antioxidant activity, βcarotene, flavonoids, Matricaria chamomilla, polyphenols, PPH, tannins. 1.44 Antifungal and Antibacterial Properties of Three Medicinal Plants from Malaysia Khoo, Kong Soo 1 Gwee, Pei Shing 2 , Chen, Li Wen 2 , Tan, Syu Wei 2 , Ong, Hean Chooi 3 , and Sit, Nam Weng 2* 1 Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia. 2 Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900 Kampar, Perak, Malaysia. 3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. <strong>Abstract</strong>: The increase of opportunistic fungal infections and the escalation of bacterial resistance, particularly multi-drug resistance have seriously impacted the chemotherapeutic agents available today. Thus the search for new antimicrobial agents from natural sources such as medicinal plants becomes necessary. Plants that are consumed as vegetables or used in traditional medicine in Malaysia were investigated for this purpose. The aerial parts of Diplazium esculentum (“vegetable fern”) and Sechium edule (“chayote” or “vegetable pear”), and the fruits of Solanum muricatum (“sweet pepino” or “melon pear”) were used, and extracted sequentially using hexane, chloroform, ethyl acetate, ethanol, methanol, and water. The extracts were then evaluated, in triplicate, against a panel of 12 medically-important microorganisms (two Grampositive bacteria, four Gram-negative bacteria, four yeasts and two molds) for microbiostatic and microbiocidal activities using colorimetric broth microdilution methods. Amphoterin B and chloramphenicol was used as the positive control for fungi and bacteria, respectively. Following extraction, the total percentages of yields obtained were 1.20%, 1.84% and 3.53% (w/w, based on fresh weight) for D. esculentum, S. edule and S. muricatum, respectively. All the plants showed antifungal activity with 66% and 49% of the bioassays demonstrated fungistatic and fungicidal 23
activity, respectively. Two yeasts, Cryptococcus neoformans and Issatchenkia orientalis were found to be 100% susceptible to all the plant extracts with the MIC values ranging from 0.08 to 2.50 mg/mL. The lowest minimum inhibitory concentration (MIC) and minimum fungicidal concentration was exhibited by the hexane extracts of S. edule and S. muricatum against C. neoformans, both with values of 0.08 mg/mL. In the antibacterial screening assays, 49% of the bioassays exhibited bacteriostatic activity while only 21% of them showed bactericidal activity. The lowest MIC and minimum bactericidal concentration was recorded for the hexane extract of S. muricatum against Bacillus cereus and Klebsiella pneumoniae, both with values of 0.31 mg/mL. The susceptibility of bacteria towards the plant extracts evaluated was species-dependent, with the susceptibility indices ranging from 0% for Escherichia coli to 72% for Pseudomonas aeruginosa. The results from this study show that extracts from these plants have significant antimicrobial activity, which corroborates their use in traditional medicine. Key words: Bactericidal, bacteriostatic, broth microdilution, extraction, fungicidal, fungistatic. 1.45 Application of Citrus aurantifolia to Control Streptococcus agalactiae Infection in Nile Tilapia, Oreochromis niloticus Kitancharoen, Nilubol 1 ., Duadin, A 1 ., and Hanjavanit, Chutima 2 1 Department of Fisheries, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002 Thailand. 2 Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand. <strong>Abstract</strong>: Effectiveness of Citrus aurantifolia to control Streptococcus agalactiae infection in Nile tilapia was investigated, including its impacts on tilapia growth rate and histopathological changes. Concerning tilapia growth rate, the fish with 50±0.12 g BW were fed with pellet feeds containing 2.5, 5.0 and 10.0% of C. aurantifolia juice for 2 mo. The results showed that specific growth rate of fish fed with the 3 concentrations of C. aurantifolia juice were significantly higher than those of the control group (p0.0) 5. On investigation of diseases control, S. agalactiae KKU 02057 were intraperitoneally injected into the fish at a dosage of 0.1ml/100g fish and observed mortality for 14 d. The fish fed with C. aurantifolia juice showed significantly higher survival rate than the control group (p
Page 1 and 2: The 3 rd International</str
Page 3 and 4: المملكت األردنيت ا
Page 5 and 6: Welcome Dear Participants, It is a
Page 7 and 8: SCIENTIFIC COMMITTEE - Mohammad San
Page 9 and 10: INVITED SPEAKERS Prof. Dr. Alain Me
Page 11 and 12: 1.3 Antioxidant Activity of Thapsia
Page 13 and 14: 1.7 High Inhibition Effect on Lipid
Page 15 and 16: products are being used as a source
Page 17 and 18: Key words: Anti-microbia, antioxida
Page 19 and 20: 1.18 Preparation and Characterizati
Page 21 and 22: 1.22 Eucalyptus globulus: Polypheno
Page 23 and 24: 1.26 Antioxidant and Antiulcerogeni
Page 25 and 26: hydroxyl radical scavenging and red
Page 27 and 28: 1.34 Anti-Inflammatory Effects of H
Page 29 and 30: 1.38 Evaluation of the Antidiabetic
Page 31: general pain. It can be used as bev
Page 35 and 36: concentrate; n = 111 versus 10 %; n
Page 37 and 38: highest TPC was observed in HZ ecot
Page 39 and 40: 1.56 Acute and Chronic Toxicity of
Page 41 and 42: Ergosterol Found in Mushrooms and Y
Page 43 and 44: potential antimicrobial activity ag
Page 45 and 46: (11.3%). The antioxidant activity o
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Page 49 and 50: 1.75 The Effect of Aloe Vera Leaf G
Page 51 and 52: 1.79 Antioxidant Activity of the Aq
Page 53 and 54: 4 minutes in room temperature. Thir
Page 55 and 56: 1.86 Hepatoprotective and Hypolipid
Page 57 and 58: ingredient were developed. Nano-nio
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Page 61 and 62: disease induced by AlCl3 on the pyr
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Page 67 and 68: 2.9 Traditional Pharmacy - Attar's
Page 69 and 70: 2.12 Traditional Uses and Scientifi
Page 71 and 72: 2.16 Survey of Medicinal Plants Fro
Page 73 and 74: 2.20 Ethnobotanical Study of Medici
Page 77 and 78: 3.5 Comparison between Polyphenol C
Page 79 and 80: according to ISO 3632.The knowledge
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egard to isoflavone concentration a
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evaluation were included 4 species
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non-serpentine, ехеpt of content
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1 Sciences Chimiques de Rennes, Cat
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guidelines came into operation in t
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3.37 Characterization and Optimizat
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of this result, ethyl acetate extra
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3.45 Chemical Composition of The Ex
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leaves, the contents of phenolics a
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Key words: Antifungal, Armoracia ru
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Abstract: A light
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The 3 rd International</str
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4.5 Formation of Hawthorn, Crataegu
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silydanin ,) while MS media supplem
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4.13 Organic Cultivation and Use of
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4.18 Effect of Some Chemical and Bi
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cultured in vitro. The WHITE and DC
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4.25 Antioxidants In Vitro Androgen
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mortality increased from 39.66 % at
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5.7 The use of seaweed extract of P
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5.11 Antioxidant and Antifeedant Ac
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carveol and δ- selinene, but inhib
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inhibition 95%. In the during of bi
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(Sulzer). All experiments were cond
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capturing genetic diversity before
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7.5 Heavy Metal Content in Hypericu
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8.3 Development of Medicinal Plants
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8.7 Industrial Products from Indige
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evaluation improvement (P
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products. The applied mulberry frui
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9.10 The Importance of Gentiana lut
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10.4 Comparative efficacy of variou
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10.8 Effect of Rhus coriaria L. in
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perforatum L.), sage (Salvia offici
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10.15 Comparative Supplemented Vita
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A. DJEBARA (66) A. John De Britto (
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Eftimova Jarmila (58) EL Tahir A (1
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Mushtaq Ahmad (143) N. Gourine (35)
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Zinchenko, Iryna (52) ZRIRA Saadia
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