Abstract Book - 3rd International Symposium on Medicinal Plants ...

evaluation were included 4 species of D. kaki L.f., D. virginiana L. (male - m and female - f
genotypes), D. lotus L. (male – m and female - f genotypes), and an interspecies hybrid D.
virginiana L. x D. kaki L.f. All tested plants were grown in the Botanical Garden of M. M. Grishko of
the National Academy of Sciences in Kiev, Ukraine. In the withdrawn leaves and defoliated oneyear
old offshoots were determined the ascorbic acid content and their dry-weight in one-month
intervals. With leaves were controlled their length and width as well. Medium length of the leaf
blade ranged from 120.19 (D. virginiana L.- m) up to 190.94 mm (D. virginiana L.) and the width
from 50.17 mm (D. virginiana L.- m) to 100.12 mm (D. virginiana L. x D. kaki L.f). Using the method
of time-interval the ascorbic acid medium increment coefficient in the leaves has been detected in
ranges from -0.19 (D. virginiana L.) up to -0.41 (D. kaki L.f.) and in the case of offshoots from -0.34
(D. virginiana L.) to -0.54 (D. virginiana L.-m). Leaves medium growth rate varried between 58.87
(D. kaki L.f.) and 80.07 % (D. virginiana L.) and for offshoots 45.44 (D. virginiana L.) and 65.19 %
(D. virginiana L.). Among the tested species, significant differences were found in the antioxidation
activity as well Leaves and ofshoots are important products, which could be utilized as resources
of biologically active substances for pharmaceutical products.
Key words: Ascorbic acid, diospyros, virginiana L, lotus L, leaves.
3.21 Secondary Metabolites from Algerian Plant Atractylis flava
Haba Hamada 1 , Chabani S 1 ., Lavaud C 2 ., Harakat D 2 ., and Benkhaled M. 1
1 Department of Chemistry, Faculty of Science, Batna University, 05000, Batna, Algeria. 2 Institut de
Chimie Moléculaire de Reims, CNRS UMR 6229, BP 1039, 51097 Reims Cedex 2, France
<strong>Abstract</strong>: The plant Atractylis flava Desf. belongs to the family Asteraceae. This family is one of
the most important botanical families of plants kingdom. It contains a large number of species so
different by their vegetative appearance, approximately 25000 species, distributed in temperate
zones of the globe, Northern Africa (Algeria, Morocco and Tunisia) and in Southern Europe (Italy,
Greece, Spain and Portugal). The genus Atractylis is chemically little investigated. As such the
studies have been realized on the toxic species Atractylis gummifera, its toxicity was attributed to
atractyloside and carboxyatractyloside, two diterpenoid glucosides capable of inhibiting
mitochondrial oxidative phosphorylation. Triterpenoids and flavonoids were isolated previously
from this genus. Atractylis flava Desf. (syn. Atractylis carduus (Forsk.) Christ.) known as yellow
thistle, is a perennial plant growing in the Saharan region. Atractylis plants have been used in folk
medicine to treat circulatory disorders, intestinal parasites, ulcers, and snake-bite poisoning.The
present work describes the isolation by chromatographic methods of eight secondary metabolites
from ethyl acetate extract of A. flava; three flavonoids: chrysin, quercetin 5,7,4‟-trihydroxy-3‟-
methoxy-3-O-α-L-rhamnopyranosyl-(1-6)-β-D-galactopyranosylflavonol, three triterpenes: oleanolic
acid, hedragenin acid and 3β,22β-dihydroxyolean-12-en-28-oic acid and two steroids: β-sitosterol
glucoside and stigmasterol glucoside. Their structures were determined by spectroscopic analysis
including 1D and 2D NMR ( 1 H, 13 C, COSY, TOCSY, HSQC, HMBC, NOESY) and mass
spectrometry (ESI-MS) and comparison with literature data. In the biological part, crude extracts
(dichloromethane, ethyl acetate and n-butanol) of A. flava exhibit antibacterial activity against
Gram positive and negative strains: Staphylococcus aureus ATCC23, Staphylococcus aureus
ATCC582, Pseudomonas aeruginosa ATCC 374 and Pseudomonas aeruginosa ATCC 53. The
anti-oxidant activity was estimated on the extracts by the DPPH test.
Key words: Algerian plant, Atractylis flava, flavonoids, secondary metabolites, species.
3.22 A flavone glucoside from the roots of Salvadora persica (Rutaceae)
Hassan Abdalla Almahy
Chemistry Department, Faculty of Science and Education, Taif University, Kingdom of Saudi
Arabia
<strong>Abstract</strong>: A flavone glucoside, luteolin 8-C-ı-d-glucopyranoside was isolated from the ethyl
acetate extract of the roots of Salvadora persica and separated using column chromatography
techniques. The structure of the compound was established by UV, IR, 1 H-NMR, 13 C-NMR, COSY,
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