INTRODUCCIÓN: REVISIÓN CRITICA DEL PROBLEMA

CAPÍTULO IX
gHSA-induced ROS production. The inhibition of NF-κB transcription factor and
PI3K tended to block the gHSA-induced ROS enhanced production, but the
inhibition was not statistically significant. Interestingly, the AP-1 inhibitor,
SP600125, in the presence of HSA significantly increased ROS production in
comparison with HSA alone (1.15 ± 0.06 for HSA+SP, p0.05, n=5; Figure 3).
These results confirmed the enhanced extracellular ROS production induced by
gHSA 25 μg/mL at 4 hours and showed an increment of ROS release in the
presence of AP- 1 inhibitor SP600125 with HSA 25 μg/mL treatment, but not with
the combination of gHSA and SP600125. In summary, only AP-1 seemed to
regulate ROS producti on and this regulation produced, at least in the presence of
HSA, an increment of the extracellular ROS production in HUVEC. NF-κB and
PI3K showed a non-statisticaltendency to regulate gHSA-induced ROS production.
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