INTRODUCCIÓN: REVISIÓN CRITICA DEL PROBLEMA

d) Effects of gHSA and SP600125 on eNOS expression.
ANEXOS
Nitric oxide (NO) produced by eNOS can react with superoxide anion and eNOS
uncoupling can produce superoxide anion instead of NO 359 . Therefore, to assess
the possible influence of eNOS on ROS production we have analysed its
expression after SP600125 treatment in the presence of gHSA or HSA.
The eNOS expression was not changed in the presence of gHSA 25 μg/mL with
respect to HSA 25 μg/mL (1.09 ± 0.05 eNOS fold change for gHSA, p > 0.05, n =
5; Figure 4). However, SP600125 showed a significant decrease on eNOS
expression both in the presence of HSA (0.51 ± 0.09 eNOS fold change for
HSA+SP600125, p < 0.05, n = 5; Figure 4) and in the presence of gHSA (0.46 ±
0.08 eNOS fold change for gHSA+SP600125 vs. 1.09 ± 0.05 eNOS fold change
for gHSA, p < 0.05, n = 5; Figure 4).
To summarize, gHSA 25μg/mL alone did not change eNOS expression levels,
whereas the AP-1 inhibitor SP600125 significantly decreased eNOS expression
independently of HSA and gHSA.
265