INTRODUCCIÓN: REVISIÓN CRITICA DEL PROBLEMA

CAPÍTULO IX
e) Effects of NOS activity on ROS production induced by gHSA.
As we have commented above, eNOS can be a source of superoxide anion when
it is uncoupled 7 . Therefore, eNOS down-regulation by SP600125 would decrease
ROS production if eNOS was uncoupled. On the contrary, down-regulation of
wellfunctioning eNOS will cause an increase in superoxide anion biodisponibility
(enhanced ROS production).
In order to determine the possible eNOS uncoupling in HUVEC, we measured the
extracelular ROS production after treatment with HSA or gHSA (25 μg/mL) during
4 hours in the presence of the eNOS inhibitor L-NAME (100 μM). L-NAME
(100μM), in the presence of HSA (25 μg/mL), induced a significant increment in
ROS production (enhanced cytochrome c reduction), similar to that observed with
gHSA (25 μg/mL) alone (1.06 ± 0.02 for HSA+L-NAME, p0.05, n > 7; Figure 5).
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