autologous blood and marrow transplantation - Blog Science ...

Lazarus et al. 155
Table 2. Effect of overnight storage on PBPCs before and after CD34 +
Before CD34 +
cell selection
Mean ±SD or median (range)
selection
Immediate Overnight
processing storage P value
Nucleated cells X10 8
/kg 7.8 (3.3-19.3) 6.7 (3.0-22.1) 0.659
% CD34 +
cells 2.0 ± 2.2 2.1+2.6 0.815
CD34 +
cells X10 6
/kg 10.9(1.5-136.1) 11(0.1-97.8) 0.812
After CD34 +
cell selection
Nucleated cells X10 6
/kg 5.5(2.8-15.5) 7.9(1.6-16.3) 0.473
% CD34+ cells 66.3 ± 19.7 60.0+18.4 0.063
CD34+cells X10 6
/kg 3.8(1.3-12.1) 4.1 (1.5-13.5) 0.847
Enrichment 55.7 (6.0-799) 52.9 (5.9-159.9) 0.315
Yield 43.6 (0.2-1205) 39.1 (8.6-80.3) 0.339
Data shown are for 30 patients (74 leukaphereses) in the immediate processing group and
for 23 patients (62 leukaphereses) in the overnight storage group. The number of nucleate
cells and CD34* cells are the results of the total of all leukaphereses administered to the
patients. Results for purity, enrichment, and yield are presented for each product
processed. Because of missing data, the n for some cells does not equal the number of
patients or the number of leukaphereses.
products (obtained from 23 patients) stored overnight. After storage and before
selection, the number of mononuclear cells, percent CD34 +
cells, and total number
of CD34 +
cells in the product did not differ significantly between groups (Table 2,
/ >
>0.659). After selection, the number of mononuclear cells, percent CD34 +
cells,
and the total number of CD34 +
cells in the product, as well as the enrichment and
yield of the selection process, were similar for those patients whose product was
stored overnight compared with patients whose product was not stored overnight
(Table 2, P>0.063).
Hematopoietic engraftment by enriched peripheral blood CD34+ cells
Hematopoietic engraftment also did not differ significantly for patients whose
leukapheresis products were stored overnight compared with those patients whose
collections were processed immediately. Blood neutrophil count reached 500/uL in
a median of 10.5 days (range 9 to 14) after immediate processing compared with
11 days (range 9 to 16) after overnight storage (P=0.421). Platelet count exceeded
20,000/uL for 7 consecutive days without a transfusion in a median of 13.5 days
(range 8 to 53) after immediate processing compared to 13 days (range 7 to 43)
after overnight storage (P=0.933, Table 3). Delay in platelet engraftment (over 21