autologous blood and marrow transplantation - Blog Science ...

586 Chapter 10: Graft Manipulation
MONONUCLEAR CELLS INFUSED/KG BODY WEIGHT
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BM PBSC
STEM CELL SOURCE
BM & PBSC
Non-Outlier Max
Non-Outlier Min
75%
25%
Median
Outliers
Extremes
Figure 1. Mononuclear cells infused per kilogram of patient body weight. Mononuclear
cells per milliliter were determined using Sysmex cell analyzer, and were combined with product
volume and patient body weight to determine cells/kg. Box and whisker representation
with median, minimum, maximum, and inner quartiles gives sense of distribution.
Twenty-three patients had adequate BM harvests but inadequate PBSC harvests by
these criteria. These patients were not randomized and, instead, were reinfused with
both products. There were no patients determined to have inadequate BM harvests.
The mean number of light-density nucleated cells per kilogram of patient body
weight (Fig. 1) collected and infused (± standard deviation [SD]) was 4.7 X10 8
(±1.8X10 8
) for patients who were randomized to receive PBSC (n=22) and
3.8X 10 s
(±1.5 X10 8
) for patients who were randomized to receive BM (n-25). The
pairwise comparison yielded P=0.0531. By contrast, patients whose PBSC
collection was determined inadequate for transplantation, and who therefore
received both products (n=23), were infused with 7.2X10 8
(±3.5X10 8
) light-
density nucleated cells per kilogram. Testing for significance of the differences
reflected by the values for all three groups using the Kruskal-Wallis ANOVA
yielded P=0.0001. Thus, while the total number of cells infused in the groups
randomized to PBSC or BM was, essentially, not significantly different, the
number infused in the group receiving both products was significantly greater than
for either the patients receiving BM or PBSC alone.
The mean number of CD34 +
cells per kilogram of patient body weight (Fig. 2)
collected and infused was 1.4X 10 6
(+1.0X 10 6
) for patients randomized to receive
PBSC and was 2.3X10 6
(±1.6X10 6
) for patients randomized to receive BM
(pairwise i°=0.0654). For the patients whose PBSC collections were inadequate