autologous blood and marrow transplantation - Blog Science ...

Janssen et al.
CD34+ CELLS INFUSED/KG BODY WEIGHT
BM PBSC BM & PBSC
STEM CELL SOURCE
~T~ Non-Outlier Max
Non-Outlier Min
• 75%
25%
• Median
* Extremes
Figure 2. CD34+ cells infused per kilogram of patient body weight. CD34 +
fraction was
determined as described in text, and multiplied by mononuclear cells/kg to yield CD34 +
/kg
and who, thus, were infused with both products, 2.4 X10 6
587
(±1.7X 10 6
) CD34 +
cells
were given. Testing for significance of the three-way difference by Kruskal-Wallis
ANOVA yielded P=0.0639. The patients infused with both products received the
same number of CD34 +
cells as did the patients receiving BM alone.
The mean number of CFU-GM per kilogram of patient body weight (Fig. 3)
collected for patients who were randomized to receive PBSC was 1.3X10 4
(±1.4X10 4
), and the number of CFU-GM per kilogram collected for patients
randomized to receive BM was 2.2X 10 4
(±1.9X 10 4
). By pairwise analysis this was
not significant, with / >
=0.0842. The patients who were not randomized but who
received both products due to inadequate PBSC collection were infused with
1.7X10 4
(±1.6X10 4
) colony-forming cells per kilogram. Testing for significance of
the three-way difference by Kruskal-Wallis ANOVA also yielded no significance,
with ^=0.1645. The patients receiving both products received the same number of
CFU-GM as did the patients receiving PBSC alone.
Granulocyte and platelet recoveries
For patients conditioned with ICE, MITT, or TNT, the median time to ANC
>500/uL was 16 days after infusion with PBSC (n=22) and 18 days after infusion
with BM (n=25) (Fig. 4). Comparing the two groups by log-rank test produced
P=0.44631. In contrast, the group of patients who received infusions of both
products due to inadequate PBSC collection experienced a median of 22 days to