autologous blood and marrow transplantation - Blog Science ...

Table 3. Immunophenotype of CD34 +
Voso et al. 567
cells
Before After P
CD34 +
/HLA-DR- 0.78 ± 0.2 0.63 ±0.1 0.2
CD34+/CD38" 0.41 ± 0.2 0.54 ± 0.4 0.2
CD34 +
/Thy-1 +
CD34 +
/Leu8 +
50.5 ±4.5 38.2 ±4.4 0.002
83.7 ±4.9 70 ± 7.5 0.014
Viability 99.2 ± 0.2 98.7 ±0.1 0.03
Hematologic reconstitution
Selected CD34 +
cells were used for the support of 13 high-dose therapy cycles
(Table 3). The remaining 18 patients had not been transplanted at the time of this
report. On average, a mean number of 7.4 ± 1.2X10 6
CD34 +
cells/kg were
autografted. As assessed by PI exclusion, the mean viability of the CD34 +
cells
after thawing was 93 ± 1.87%. The patients engrafted with a mean time to
leukocyte (1.0X10 9
/L), neutrophil (0.5X10 9
/L), and platelet (20.0X10 9
/L)
recovery of 12.8 ± 0.7, 13.6 ± 0.7, and 14.5 ± 1.3 days, respectively (Fig. 2).The
patients remained in hospital for a median of 15 days (range 12-21) after
autografting and had a median of 3 days of fever (range 1-10). They required a
median of six platelet and four erythrocyte transfusions (range 2-10 and 2-8,
respectively).
The engraftment ability of the CD34 +
autografts was compared with that of
unmanipulated PBSC autografts containing 9 ± 0.7 X10 6
CD34 +
cells/kg which
were used for the support of high-dose therapy in 104 patients. The mean time
Days
10 15 20 25
to PMN > 0.5 x 10VL
30 35 0 10 20 30 40 50
to Platelets > 20 x 10VL
Figure 2. The hematopoietic reconstitution ability of the selected CD34+ cells (closed s
bols) is similar to that ofCD34* cells contained in unmanipulated autografts (open symbo
The time to recover leukocytes to 1X10 9
/L, neutrophils to OSXltf/L, and platelets to
20X10 9
/L was 12.8 ±0.7vs. 12.6 ±0.3days (P=0.8), 13.6 ±0.7vs. 13.9 ±0.4days (P=0.8),
and 14.5 ±1.3 vs. 13 ± 0.8 days (P=0.6), respectively.