PDF file: EURASNET Annual Report 2008

overexpressor and mutant lines(month 42)185 The plant groups (will establishcollaborations with other groupsto analyse proteins which affectalternative splicing (month 42)186 Relativ quantitative massspectrometricanalysis ofspliceosomal complexes. (month42)187 Establishing a map forspliceosomal proteins in a novel2D electrophorese systemwithout IEF. (month 42)188 Feasibilty studies for chemicalcross-linking of proteins withinpurified spliceosomes and/orsubspliceosomal proteincomplexes. (month 42)189 Role of Cwc21p in splicingspecificity and fidelity (month42)190 MS based proteomics of Brr2contain complexes (month 42)191 Establishing of stable cell lineL4-33K and studying cellularand viral protein–proteincomplexes containing L4-33Kincluding subcellularlocalization. (month 42)192 Characterization of proteinheteromeric complexesassociated with regulated HIVRNA splice sites. (month 42)193 Relative quantitative massspectrometry of proteinsaossociated with regulated HIVRNA splice sites, RSV RNA andwildtype/mutant exon 5 ofLMNA pre-mRNA. (month 42)90 Use the tap-tagged SRp30protein to select and MS-analyzeribonucleoprotein complexes innSBs (month 42).continued194 Bertrand will analyze in detailthe binding of U1-specificproteins to the transcription siteof the HIV-1 reporter RNA byFRAP, including (i)interpretation of the FRAPcurves with a diffusion-reactionmodel, (ii) analysis of thebinding of U1 proteins onreporter RNA containingmutation in their splice donorsequence (either stabilizing ordestabilizing U1 binding), (iii)analysing the binding of mutantproteins, unable to bind U1snRNP or other partners. He willdetermine the recruitment ofsplicing factors characteristic ofa given spliceosomal complex8 42 42 Smith9 42 36 Lührmann9 42 36 Lührmann9 42 36 Lührmann9 42 36 Lührmann9 42 39 Lührmann9 42 partially delayed Lührmann9 42 36 Lührmann9 42 36 Lührmann10 42 on target (42) Cáceres10 42 on target (42) Cáceres101