PDF file: EURASNET Annual Report 2008

Participant 2 – Juan Valcarcela) Work performed during the period• WP2• WP3• WP5• WP9• WP10• WP11• WP14• WP15• WP17• WP18• WP20Shared resources, technology and reliable protocols related to splicing-sensitive microarrays.Mentoring of EURASNET YIPsManagement of Splired, the Spanish network of transcriptomics. Part of application forfunding of splicing-sensitive microarray work in a systems biology framework for breastcancer.Characterized the role of spliceosomal components and their complexes (U2AF, SPF45,RBM5, TIA-1 / TIAR, EWS, PTB, Raver-1) in alternative splicing.Continued characterization of signaling route leading to regulation of splicing factor TIA-1and its target gene Fas.Led work in this workpackage and contributed splicing microarray experiments in Drosophilaand mammalian cells. Characterized the function of TIA-1 isoforms as wll as changes in ASin 3’ UTR regions.Collaborative work to explore transcriptome changes under conditions of reduced elongationrate of RNA pol II and upon UV irradiationParticipated in the design and set up of splicing microarray validation experiment as well asdeep sequening technologies and analysis tools. Contributed to the development of thesetechnologies.Host to two collaborative exchanges with other EURASNET groups.Provided advice to PhD students and postdocs from EURASNET labs on career moves.Contract with Marcelino Botín Foundation for IP protection and commercialization of theresearch activities of the group.133