PDF file: EURASNET Annual Report 2008

296 Group 12a (Branlant) willuse the method developed incollaboration with A vandorsealler (Strasbourg) forstudy of native RNPcomplexes reconstituted fromin vitro transcribed RNAs andpurified recombinant splicingfactors.297 Group 17 (Eperon) willdevelop the use of singlemolecules methods to followthe stoechiometry of factorsin splicing complexes andstart to build a new TIRFsystem to encompass the useof more laserssimultaneously.298 Group 17 (Eperon) and 23(Smith) will continue tostudy binding of protein PTBon alpha-tropomyosin premRNAat the level of singlemolecule.299 Group 29 (Allain) willinvestigate the structure ofCUG-BP2 and SC35(collaboration with J.Stévenin) in complex withRNA300 MJAY analysis of P19 cellsin which SRp20 and/orSRp75 have been depleted byshRNAs (month 54,Neugebauer).301 Further testing of ability ofSolexa sequencing to providequantitative profiling ofalternative splicing,incorporating reads that mapto exon-exon junctions(month 48, Smith, Valcarcel)302 Continue to exploit the ASRT-PCR panel to analysefunction of splicing andRNA-interacting factors fromEURASNET members andother plant scientists (month54)303 Examine the utility of usingnew generation sequencing toidentify and quantifyalternative splicing in plants(month 54)304 Genomic SELEX for RNAsbinding to plant splicingfactor (month 54)305 Isolation of selected samplesof regulated and semiquantitativecharacterizationof their proteins.7 Branlant O CO 547 Branlant O CO 547 Branlant O CO 547 Branlant O CO 548 Smith O CO 548 Smith O CO 548 Smith O CO 548 Smith O CO 548 Smith O CO 549 Luhrmann O CO 54241