PDF file: EURASNET Annual Report 2008

Participant 10B – Glauco Tocchini-Valentinia) Work performed during the period• WP210B participated in WP-specific networking activities and overall projectmanagement and coordination activities• WP810B developed novel applications of the original cis- and trans- Archaea-expresstechnology for analysis of alternative splicing of biomedically relevant membrane receptor genetranscripts, in mouse cell lines and other eukaryotic model systems (S. cerevisiae).• WP1110B carried out experiments with application of the cis- and trans- Archaea-expresstechnology (Deidda et al., 2003; Tocchini-Valentini et al., 2005-2007), to characterize and selectivelymodify, in vitro and in vivo, components of genetic circuits involved in alternative splicing of targetmRNas, in mouse cell lines and tissues.• WP1410B applied specific experimental procedure for the development of novel cellularassays of modulators of alternative mRNA splicing, using yeast (S. cerevisiae) and mouse cultured cellmodels.• WP1510B designed and produced novel construct and expression vectors, for theexperimental identification and analysis, in eukaryotic S. cerevisiae cells, of specific cis- and transsplicingevents, mediated by tRNA sequences.• WP1710B participated in WP-specific management and integration activities.• WP1810B participated in WP-specific management and integration activities• WP2010B participated in WP-specific management and integration activitiesb) Major cost items with justificationParticipant 1A Type of expenditure Budget total costspersonnel 66.043,25consumables 0training 0equipment 0travel 1.960,57= total direct costs 68.003,82+ overhead 52.504,38adjustment -3.472,70= total eligible costs 117.035,50152