PDF file: EURASNET Annual Report 2008

338 Role of the U1 snRNP in“reverse coupling” of HIVtranscription and splicing.339 Establishment of “modelgenes” to monitor cotranscriptionalspliceosomeassembly in mammalian cellsin vivo.340 Feasibility study, targetingPTB regulation of AS tomodel genes by introductionof MS2 stem loops in intronicpositions (replacesdeliverable 218)341 Measure splicing rates in realtimewith the MS2-GFPtechnique.342 Tazi’s group will continue toscreen novel synthesizedmolecules on luciferasereporter which reproducesthe effect of the LMNAmutation on splicing and exonskipping of exon 51 ofDystrophin. Stable cell linesexpressing this reporter areused to screen splicinginhibitors either alone or incombination with AAVvectors harbouring U7antisense that mask themutated sequence.343 Tazi’s group in collaborationwith Splicos, a start upcompany, is setting up ascreen of splicing inhibitorsthat prevent cancer cellmotility in 2-D and 3-D setup.344 Ast’s group will screenadditional molecules thataffect the splicing pattern ofthe IKAP mRNA in FD cells.In addition, a foodsupplement was also found toaffect the IKAP mRNA, andcan be used as a safe,potential drug.13 Neugebauer O CO 5413 Neugebauer O CO 5413 Neugebauer O CO 5413 Neugebauer O CO 5414 Tazi O CO 5414 Tazi O CO 5414 Tazi O CO 54248