PDF file: EURASNET Annual Report 2008

288 Groups 12a (C Branlant) willcontinue to identify proteinsinvolved in regulations ofHIV-1 RNA, SC35 and laminA pre-mRNA splicing incollaboration with Groups 25(Stévenin), Group 12a(Tazi), Group 19 (Kjems)and Group 1 (Lührmann).Study on the role of MBNL1and the possible involvementof other splicing regulatoryfactors on the regulation ofhcTNT exon 5 inclusion, inlink with DM1, will becontinued.289 Group 19 (Kjems) willcomplete the search forfactors involved in regulationof MCAD exon 5 utilization.290 Group 7 (F Baralle) willcomplete the study ofinteraction of splicingregulatory factors withCERES elements.291 Group 12c (Branlant) incollaboration with Group 10a(Biamonti) will study theinteraction of sat III RNAwith ASF/SF2 and othersplicing regulatory factors.292 Group 12c will continue tostudy how MBNL1 interactswith CUG and CCUGrepeated sequences and theinfluence of other nuclearfactors on this binding. Bothexperimental and computerbased 3D structure analysesof the interaction will beachieved.293 Group 12a (Branlant) willstudy binding of MBNL1 toits normal regulatoryelements in hcTNT and Taupre-mRNAs by site-directedmutagenesis, footprintingassays, FRET experimentsand native mass spectrometry.294 Group 23 (Smith) willperform deletion and pointmutations to define theminimal repressor domain ofMBNL1 by MS2 tetheringdownstream of Tpm1 exon 3.The defined domain will beanalyzed for interaction withother proteins and with RNA.295 Group 12a will made anextensive analysis of hnRNPK and hnRNP A1 binding onHIV-1 splicing regulatoryregions using the sameapproach as for MBNL1.7 Branlant O CO 547 Branlant O CO 547 Branlant O CO 547 Branlant O CO 547 Branlant O CO 547 Branlant O CO 547 Branlant O CO 547 Branlant O CO 54240