PDF file: EURASNET Annual Report 2008

Participant 21 – Angela Krämera) Work performed during the period• WP2Provided EURASNET members with antibodies and plasmids• WP8– Optimization of CLIP of SF1 and U2AF65 for high-throughput sequencing– Analysis of SF1 in AS of UPF3a pre-mRNA (identified as a SF1 target by CLIP)• WP11– Cataloguing SF1 isoforms in HeLa cells and mouse tissues by Western blottingantibodies against different SF1 epitopes– Generation of antibodies against additional SF1 isoforms (Dundee Cell Products)– Identification of immunoprecipitated SF1 isoforms and SF1 interacting proteinsby mass spectrometry (in collaboration with Henning Urlaub)• WP16– Co-organiser of the “First International EURASNET Conference on AlternativeSplicing” in Krakow, May 21-23, 2008– Attendance of EURASNET conferences, workshops and IFMs as detailed below(see “Travel”)• WP17Coordination of staff exchange and training• WP21Co-organizer (together with Daniel Schümperli and Oliver Mühlemann) of theAnnual Swiss RNA Workshop in Bern, to be held on January 30, 2009. Thisworkshop is sponsored by the Swiss Committee for Molecular Biology (funded bythe Swiss National Science Foundation), with ca. 120 participants, 14 short talksselected from submitted abstracts, 2 talks from invited speakers (Benoit Chabot andMatthias Hentze) and poster sessions.• WP22Participation in telephone conferences and discussions of management membersb) Major cost items with justificationParticipant 1A Type of expenditure Budget total costspersonnel 8,647.07consumables 684.70trainingequipmenttravel 1,344.84= total direct costs 10,676.61+ overhead 2,135.32= total eligible costs 12,811.93Major cost items177