PDF file: EURASNET Annual Report 2008

Participant 11 – Albrecht Bindereifa) Work performed during the period• WP2Added our splicing constructs to list of reagents.• WP8Completion of detailed mutational analysis of several new in vivo targets of hnRNPL, human TJP1, SLC2A2, and ITGA1, that came out of our exonarray analysis(Monika Heiner and Albrecht Bindereif, manuscript in preparation). In addition, thehnRNP L pre-mRNA itself was characterized as an unusual target of the hnRNP Lprotein, creating an autoregulatory mechanism [Rossbach, Hung, Schreiner, Grishina,Heiner, Hui, and Bindereif (2008). Auto- and crossregulation of the hnRNP Lproteins by alternative splicing. Mol.Cell.Biol., in press].• WP12Continuation of characterization of human factor p110 and its role in U4/U6 snRNPrecycling. Shown in vitro that these proteins bind synergistically to U6 snRNA andthat both purified and recombinant LSm2-8 proteins recruit p110 protein to U6snRNA via interaction with the highly conserved C-terminal region of p110 [Licht,Medenbach, Lührmann, Kambach, and Bindereif (2008). 3’ Cyclic Phosphorylationof U6 snRNA Leads to Recruitment of RecyclingFactor p110 through LSm Proteins. RNA 14, 1532-1538].In addition, a new collaboration developed with the Israeli group of Eli Sprecher, adermatologist working on various rare diseases. Specifically, we have contributed tocharacterize the molecular consequences of the ANE syndrome, which is based on amutation in the human RBM28 gene [coauthorship: Nousbeck, Spiegel, Ishida-Yamamoto, Indelman, Shani-Adir, Adir, Lipkin, Bercovici, Geiger, van Steensel,Steijlen, Bergman, Bindereif, Choder, Shalev, and Sprecher (2008). Alopecia,neurological defects, and endocrinopathy syndrome caused by decreased expressionof RBM28, a nucleolar protein associated with ribosome biogenesis. Am. J. Hum.Genet. 82, 1114-1121]. We are continuing to analyze how the deficiency of theRBM28 protein affects the splicing machinery.• WP19Visits of four high-school pupils in our lab, each of them for 2 weeks in 2008, to getintroduced into biochemical and splicing research.Hold two seminars on general introduction into splicing research for associations ofGraduate students at Giessen University.b) Major cost items with justificationParticipant 11 Type of expenditure Budget total costspersonnel 33,054.83consumablestrainingequipmenttravel= total direct costs 33,054.83+ overhead 6,610.97= total eligible costs 39,665.80Major cost items• ResearchPersonnelInna Grishina [WP 8, 19] 1,360 hours 20,150.- EuroSilke Schreiner [WP 8, 19] 300 hours 7, 450.- EuroMonika Heiner [WP 8, 19] 300 hours 5,450.- Euro154