PDF file: EURASNET Annual Report 2008

345 Schümperli’s group isplanning to test the inducibleU7 system by transgenesis inthe SMA mice and tointensify - throughcollaborations - efforts to finda somatic delivery route forthe therapeutic U7 cassetteand to improve its expressionin mouse cells and tissues.Additionally this group willanalyse the global changes insplicing and proteinexpression induced in humanSMA patient fibroblasts orSMA mouse spinal cord andtheir modulation by thebifunctional U7 cassette.346 Eperon’s group is planningto test a subset of modifiedTOES oligonucleotides inSMA mice as drug-likestimulators of specificsplicing events.347 Eperon's group just startedin collaboration withBaralle’s group toinvestigate the possibility ofusing TOES to stimulatesplicing of exons damaged bymutation in cystic fibrosisand of undamaged skippedexons in genes with roles inthe development ofleukaemia.348 Tazi’s group in collaborationwith Splicos is planningvalidation of selectedmolecules in mice models forHGPS, DMD and inhumanized mouse model thatare reconstituted with PBMCsand infected with HIV 1349 Extend Crosslinking studiesto other RRM containgproteins that are involved inalternative splicing.(continuation of 232)350 ESI-MS detection ofcrosslinks will be applied tothe investigation of trisnRNPsand spliceosomalcomplexes purified fromHeLa and yeast.351 Continue and further developthe use of travel bursaries forstudent and postdocexchanges for training andparticipation at intraworkpackagemeetings (40PMT/18 month) (month 42)(continuation of deliverable237)14 Tazi O CO 5414 Tazi O CO 5414 Tazi O CO 5414 Tazi O CO 5415 Seraphin O CO 5415 Seraphin O CO 5417 Krämer O CO 54249