PDF file: EURASNET Annual Report 2008

Participant 26 – Didier Auboeufa) Work performed during the period• WP2Exchange of protocols and technical expertise in molecular biology and analysis of microarrays.• WP4Create an interface that allows direct visualization of splicearray data.• WP8Transcriptome analysis of four different mouse primary mammary tumors that show aspectrum of metastatic phenotypes using the Affymetrix Exon array system coupled with a manualannotation of alternative exons either as alternative first exons, alternative last exons or alternativespliced exons.• WP13Analysis of co-transcriptional splicing on specific genes in the Ewing Sarcoma and MCF-7breast cancer cell models.• WP21Participation in local and national programs with the specific view of raising awareness aboutalternative splicing in cancer.b) Major cost items with justificationParticipant 26 Type of expenditure Budget total costspersonnel 3 329,89consumables 53 415,96training 0equipment 0travel 6 662,27= total direct costs 63 408,12+ overhead 12 681,62= total eligible costs 76 089,74Major cost items• ResearchPersonnel : 3 329.89 EurosGabriel Sanchez (WP13) : Co-transcriptional splicingConsumables : 53 415,96 EurosChemicalsEnzymes, Bio MolqPCRLaboratory suppliesCell cultureAffymetrix Exon ArraysAntibodies, ChIP4 878,92 Euros25 844,94 Euros5 600,20 Euros3 543,93Euros2055,83 Euros7 056,20 Euros4413,18 EurosTravel: 6 662.27 Euros- EURASNET Montpellier meeting, 17-20 February 2008, Montpellier, France- RNA meeting, 28 july-03 August 2008, Berlin, Germany- EURASNET Krakow meeting, 21-25 Mai 2008, Krakow, Poland- SFBBM-sifrARN, 10-12 September 2008 La Grande Motte, France185