PDF file: EURASNET Annual Report 2008

163 The Eyras group incollaboration with the Smithgroup will study the splicingregulation of introns in whichthe branch site sequencelocated more than 100nucleotides from the 3’ss.They will use comparativemethods to extract possibleregulatory sequences andsecondary structres that maybe involved in the regulation.They will also identify thecases that are related todisease. In colaboration withthe Ast group they willcompare the splicingmechanism in eukaryotes.Using multiple eukaryoticspecies, they will compare theproperties of protein factorsand snRNAs involved insplicing. They will investigatethe role of the SR andhnRNPs proteins in the originof regulated splicing (month42).176 Exon Array (and junctionarray, if available) microarrayanalysis of effects of SF1knockdown upon alternativesplicing (Krämer, month 42)180 Application of deepsequencing to CLIP tags ofSF1 and U2AF65 (Krämer,month 42)184 Continue to completeanalyses of Arabidopsis NMDmutants and hyl1; SR proteinoverexpressor and mutantlines (month 42)185 The plant groups (willestablish collaborations withother groups to analyseproteins which affectalternative splicing (month42)190 MS based proteomics of Brr2contain complexes (month42)191 Establishing of stable cell lineL4-33K and studying cellularand viral protein–proteincomplexes containingL4-33K including subcellularlocalization. (month 42)90 Use the tap-tagged SRp30protein to select and MSanalyzeribonucleoproteincomplexes in nSBs (month42).continued6 Ast O CO 428 Smith O CO 428 Smith O CO 428 Smith O CO 428 Smith O CO 429 Lührmann O CO 429 Lührmann O CO 4210 Cáceres O CO 42232