PDF file: EURASNET Annual Report 2008

Participant 1C – Henning Urlauba) Work performed during the periodWP2: Establishing protocols for the metabolic chemical stable isotope labeling of proteins in HeLa cells and thesubsequent generation of splicing active nuclear extract for the in vitro assembly of differentially labeledspliceosomal complexes.WP15: Establishing a rapid and user friendly protocol for the mass spectrometric analysis of protein-RNAcontact sites in UV-light irradiated ribonucleoprotein (RNP) complexes. Establishing electrospray ionizationmass spectrometry for the sequence analysis of crosslinked peptide-RNA oligonucleotide conjugates.Identification of novel, hitherto unknown contact sites in U1 and 12S U2 snRNP RNP complexes, comprisingand extending the RNA recognition motif in the U1-specific 70K protein, the zinc finger motif in the U1 Cprotein and the Sm domains in the U1 and U2 Sm proteins.b) Major cost items with justificationParticipant 1A Type of expenditure Budget total costspersonnel 19.966,12consumables 977,15trainingequipmenttravel 59,72= total direct costs 21.002,99+ overhead 4.200.60= total eligible costs 25.203,59Major cost items• ResearchPersonnelFlorian Richter [WP 15] 12 PM 19.966,12 EuroConsumablesChemicals977,15 EuroFlorian Richter is a PhD student working on the development of cross-link identifications betweenproteins and RNA via mass-spectrometry (WP15).Consumables include general lab chemicals and minor replacement parts for mass spectrometrymachines.132