PDF file: EURASNET Annual Report 2008

trans-acting factors of particularinterest with regards to theirfunctionality, biochemicalproperties, and generalimportance for the cellularmetabolism particularly in thedevelopment of tumors(Biamonti) (month 42)212 Development ofoligonucleotide-based or snRNPbased approaches to correctsplicing defects with specialemphasis on investigating themolecular mechanisms affectedby these novel reagents. (month42)213 Intensification of the interactionsamong medically interestedpeople of EURASNET creatingspecial interest groups centeredon common clinical problemsand diseases. (month 42)214 Further development oftechniques for use in diagnostictests including the standardquality assurance required fordiagnostic molecular geneticlaboratories. (month 42)215 To determine the molecularbasis for the effect of Prp45p ontranscription, Beggs will analysethe effects of prp45 mutantalleles on the recruitment ofCTD kinases and chromatinremodeling factors to the ASC1gene by in vivo ChIP assay, andthe interaction of these proteinswith Prp45p will be tested inpull-down assays in vitro.(month 42)216 Aubeouf and Neugebauer willcollaborate to establish splicingfactor ChIP in MCF7 cell lines.These breast cancer cells areresponsive to estradiol, whichstimulates transcription of cyclinD1, PS2 and other genes. Thiscellular model will allow us totest the splicing process on geneproducts made in response tohormones. We will establishstable cell lines with integratedcopies of BACs expressingGFP-tagged versions of U1 70K,U2AF65, Prp8, CBP80 andmembers of the SR proteinfamily, in order to investigatethe interplay betweentranscription, co-transcriptionalspliceosome assembly and ASoutcome. (month 42)217 Kornblihtt will determinewhether intronic siRNAs affect12 42 36 Baralle12 42 36 Baralle12 42 30 Baralle13 42 on target Neugebauer13 42 on target Neugebauer13 42 36 Neugebauer104