PDF file: EURASNET Annual Report 2008

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Author Title Publication <strong>EURASNET</strong> Public. +/- 3 <strong>EURASNET</strong>acknowledgement 4Kastner, B. , Fischer, N., Golas, M. M.,Sander, B., Dube, P., Boehringer, D.,Hartmuth, K., Deckert, J., Hauer, F., Wolf,E., Uchtenhagen, H., Urlaub, H., Herzog,F., Peter, J. M., Pörschke, D., Lührmann,R., and Stark, H.Urlaub, H., Kühn-Hölsken, E., andLührmann, R.GraFix: sample preparation for single-particle electroncryomicroscopy.Analysing RNA-protein cross-linking sites in unlabeledribonucleoprotein complexes by mass spectrometry.Nat Methods. 5:53-55.In: Methods in MolecularBiology, vol. 488: RNA-Protein InteractionProtocols. Edited by: Ren-Jang Lin, Humana PressInc., 221-245.+ -+ -119
8 TABULAR OVERVIEW OF ALL RESOURCES EMPLOYED ON THE PROJECTThe last column shows the estimate of person-months of permanent staff working on the project.No. Partner Eligible costs Non-EUfundedpersonnelcosts (€)Estimatedcosts forotherresourcesdeployed bythe project(€)Estimatedtotal costs1A MPG 308.643,07 184.000 150.000 642.643,07 491B MPG 43.538,80 113.300 70.000 226.838,80 481C MPG 25.203,59 75.000 50.000 150.203,59 482 CRG 65.996,26 140.000 220.000 419.893,81 183 UERLN 5.128,32 17.000 10.000 32.128,32 104 UU 29.863,21 56.054,47 56.000 141.917,68 55A EMBL 42.845,47 17.534,30 10.000 70.379,77 25B EMBL 86.052,66 13.770,10 10.000 109.822,76 1.56 TAU 37.703,42 120.000 95.000 252.703,42 197 ICGEB 65.841,85 112.730,31 18.533,33 197.105,49 408 MUW 63.888,58 117.000 45.000 225.888,58 69 UEDIN 41.219,01 109.892,73 31.200,00 182.311,74 29.510A CNR FC10B CNR FC11 LUG 39.665,80 130.000 100.000 269.665,80 212A CNRS FC12B CNRS FC12C CNRS FC12D CNRS FC13 UNIBE 62.836,80 117.852 56.912 237.600,80 1214 SCRI 114.865,46 85.000 14.000 213.865,46 2215 MRC FC16 IMM 47.552,10 75.000 80.000 202.552,10 4817 UNILEIC 27.811,13 32.056,47 0 59.867,60 518 AMU 85.587,11 9.604,04 25.000,00 120.191,15 919 UAAR 51.967,08 280.000 75.000 406.967,08 820A FCEN-UBA 39.701,39 80.000 187.200 306.901,39 820B FCEN-UBA 38.835,06 28.000 21.200 88.035,06 321 UNIGE 12.811,93 169.000 64.000 245.811,93 1222 UNIVDUN 65.605,43 39.960,50 20.000 125.565,93 1023 UCAM-DBIOC 36.542.63 222.903,32 57.658,90 317.104,85 6124 HUJI 20.137 60000 24000 104137 625 CERBM-GIE 52.323,78 49.980 102.303.78 1226 INSERM FCF27 FCSR 60.223,18 90.000 25.000 175.223,18 028 ISB-SIB 40.846,80 46.950,00 10.000,00 97.796,80 1229 ETHZ 7.366,37 120.000 20.000 147.366,37 030 IIMCB 18.486,41 35.132,43 6.045,05 59.663,89 931 UPF 28.380 14.029,1 9.620,90 120.000 1232 SOTON-HGD 0 17.080 16.459 33.539 1PM ofpermanentstaff (non-EU funded)120
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TABLE OF CONTENTSA. PERIODIC ACTIVI
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1 PUBLISHABLE EXECUTIVE SUMMARY EUR
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Dr. Davide Gabellini(28) Swiss Inst
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WP7 Molecular characterization of s
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To identify protein-protein interac
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expressed in a particular tissue. T
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Duchenne muscular dystrophy (DMD).
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WP21 Reachout to the broader RNA co
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4.1 TABLE OF MILESTONES JPA MONTH 2
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Numerous plasmids, constructs, extr
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collaboration between EURASNET and
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5.1 WORK PACKAGE REPORTSWork Packag
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• Ongoing regular addition of new
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Work Package 4 The Alternative Spli
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Work Package 6 In silico approaches
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alternative splicing.162 Compare sp
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33 Agreement on pre-mRNA substrates
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its numerous binding sites on both
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Work Package 8 Genome-wide Analyses
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173 Further development of data ana
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Problems and explanations for delay
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eveal that there is a dramatic chan
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Work Package 10 Post-translational
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mutational analysis that led to the
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Isolation of an active step I splic
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FBP11 and 21 with these sequences.
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Similarly to what observed for SF2/
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prp45-113 mutant, which is compatib
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cell lines. In contrast in the pres
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genes with roles in the development
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Structural analyses of several RRMs
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Work Package 17 Staff Exchange and
Page 69 and 70: Barralle's lab, Krämer is acting a
Page 71 and 72: alternative splicing) and higher ed
Page 73 and 74: Student Symposium “Decision Makin
Page 75 and 76: variation and suitability for use w
Page 77 and 78: Following feedback from members we
Page 79 and 80: Invited seminars: In addition to me
Page 81 and 82: Publications 2008Author Title Publi
Page 83 and 84: Author Title Publication EURASNET P
Page 93 and 94: Minutes of the General Assembly dec
Page 96 and 97: 241 Evaluate available careerdevelo
Page 98 and 99: 156 The EURASNET memberStamm publis
Page 100 and 101: y coarse-grained foldingsimulation.
Page 102 and 103: overexpressor and mutant lines(mont
Page 104 and 105: machinery with particularrespect to
Page 106 and 107: alternative splicing, using theEDI
Page 108 and 109: contemporary findings withinthe EUR
Page 118 and 119: Licht K, Medenbach J, Lührmann R,K
Page 122 and 123: 9 TABULAR OVERVIEW OF MAJOR COST IT
Page 124 and 125: total costs * 18.891,12 52778.60 41
Page 126 and 127: other (the rest) 48,57 0 56.539,43t
Page 128 and 129: travel 6.964,91overhead 3.081,07oth
Page 130 and 131: Participant 1A Type of expenditure
Page 132 and 133: Participant 1B - Karla Neugebauera)
Page 134 and 135: Participant 2 - Juan Valcarcela) Wo
Page 136 and 137: Participant 3 - Stefan Stamma) Work
Page 138 and 139: Participant 4 - Göran Akusjärvia)
Page 140 and 141: Participant 5A/B - Peer Bork/Rolf A
Page 142 and 143: Major cost items• ResearchPersonn
Page 144 and 145: Participant 7 - Francisco Barallea)
Page 146 and 147: Francisco Baralle gave a talk to a
Page 148 and 149: alternative splicing on human disea
Page 150 and 151: J. Beggs has had frequent contact w
Page 156 and 157: Participant 12A - Jamal Tazia) Work
Page 158 and 159: Participant 12B - Bertrand Séraphi
Page 160 and 161: Participant 12C - Christiane Branla
Page 162 and 163: Participant 12D - Edouard Bertranda
Page 164 and 165: Participant 13 - Daniel Schümperli
Page 166 and 167: Participant 14 - John W. S. Browna)
Page 168 and 169: Participant 15 - Javier F. Cáceres
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• ResearchPersonnelName WP Person
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MiscellaneousOligonucleotidesUse of
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Major cost items• ResearchConsuma
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Major cost items• ResearchPersonn
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Participant 21 - Angela Krämera) W
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Participant 22 - Angus Lamonda) Wor
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Participant 23 - Chris Smitha) Work
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ChemicalsEnzymesLaboratory supplies
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Participant 26 - Didier Auboeufa) W
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tool for the molecular analysis of
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Participant 29 - Frédéric Allaina
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protein and instead possesses the d
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+ overhead 4.730= total eligible co
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Claudia Tamaro (PhD) has begun work
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ETHZIIMCBUPFSOTON-HGDTOTALJoint Pro
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Direct eligible costs 53.240,48 0,0
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Direct eligible costs 18.720,07 0,0
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13 REPORT ON THE DISTRIBUTION OF TH
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Report on the Distribution of the C
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For most work packages here follows
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pathways (eg, Reactome ).While PAND
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The possible dependence of the effe
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In some treatments or mutants, simi
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Work Package 12: Mis-splicing and D
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acid. Furthermore, using antibodies
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At the 2007 annual meeting in Ile d
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Partner 13 has extensively studied
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inhibitors/modulators of RNA splici
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18 MONTH JPA: WORKPACKAGE TIMECOURS
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15.3 GRAPHICAL PRESENTATION OF WORK
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15.4 TABLE OF MILESTONES (MONTH 37-
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156 The EURASNET memberStamm publis
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194 Bertrand will analyze indetail
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216 Aubeouf and Neugebauer willcoll
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238 Establishing joint PhDcommittee
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283 A unified browser for allknown
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296 Group 12a (Branlant) willuse th
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315 Lamond will use a FLIM-FRET bas
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329 GROUP 7 (F. BARALLE)will contin
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333 GROUP 27 (GABELLINI)will:1) foc
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345 Schümperli’s group isplannin
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366 Quarterly network e-mail atmont
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2. Sharing Resources, Technology an
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5. Ensuring DurabilityWorkpackage d
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7. Molecular Characterization of Sp
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8. Genome-wide Analyses of Splicing
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9. Complexity of Spliceosomal Prote
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202 Srebrow will investigate the ro
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12. Mis-splicing and DiseaseWorkpac
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13. Co-transcriptional Mechanisms o
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14. Chemical Biology and Therapeuti
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15. Development of Enabling Technol
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269 IFMs in 20091. “Alternative a
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18. Career DevelopmentWorkpackage d
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20. SMEs and Technology TransferWor
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21. Reachout to the Broader RNA Com
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15.7 WORK PACKAGE LIST (MONTHS 37-5
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ETHZIIMCBUPFSOTON-HGDTOTALJoint Pro
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AMU 5 29 8 42UAAR 5 145 16 1665 29
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Joint Programme of Activities RESEA
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60 month period 18 month periodRequ
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Integration1. Young Investigator Pr
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UCAM-DBIOC 2350,000x1/3 € 50,000x
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the management team. Audit costs ar
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