PDF file: EURASNET Annual Report 2008

Participant 4 – Göran Akusjärvia) Work performed during the period• WP2Contributed plasmidsWP7Characterized the cis-acting elements in the L4 pre-mRNA activating L4-33K selfsplicing.• WP9Affinity purification of the pre-spliceosome from adenovirus-infected cells performed.Affinity selection of splicing related complexes induced by the viral L4-33K protein has beendone. The subcellular localization of the viral L4-33K and L4-22K proteins has been studied.• WP10The effect of protein kinase A on splicing in adenovirus-infected cells has been studied. Theresult shows that PKA negatively regulates viral alternative splicing by phosphorylating thekey viral protein controlling alternative RNA splice site choice.• WP11Demonstrated that the adenovirus L4 region via alternative splicing produces two relatedproteins. One protein functions as an alternative RNA splicing factor the other as a viraltranscription factor.• WP12The work related to this package now done in the context of WP11• WP18Recruited Dr. Tanel Punga as an Assistant Professor in RNA and infectious disease.b) Major cost items with justificationParticipant 1A Type of expenditure Budget total costspersonnel 18.435,12consumables 5.676,83trainingequipmenttravel 167,84= total direct costs 24.279,79+ overhead 4.855,95= total eligible costs 29.135,74137