PDF file: EURASNET Annual Report 2008

2. Sharing Resources, Technology and Reliable ProtocolsWorkpackage description (month 37-54)Workpackage number 2 Start date or starting event: Month 0Participant idAllPerson months 52.5Objectives• To create a seamless, integrated network that allows each lab to draw upon the knowledge and resources availablein other labs.• To promote and encourage shared use of already existing facilities belonging to individual institutes whenever thisis compatible with existing on site work loads and work flows.• To actively ensure transfer of a technology fully developed in one laboratory to another participating laboratorystill lacking this technology.Description of work1. Expertise, methods and facilities are available for 2D DiGE (Cy2/3/5) (CS), MALDI-TOF and MS-MS proteinmass spectrometry (CS, KN, RL, AL), monoclonal antibody production (KN), transgenic mice via direct injection orspecific targeting in ES cells (HS, IE), high throughput screening (KN), DNA sequencing (HS, AL), SNP analysis(HS), electron microscopy (HS, AL, RL), recombinant protein purification (HS), biostatistic services (HS) andmicroarray analysis (AL, JV). Expertise is also available in confocal microscopy and photobleaching, RNA-FISH,FRET and related techniques (MCF, GAs, KN, GB, HS), minigene analysis (SS, GAs, GB), microarray analysis ofmRNA isoforms (JV) and mRNA from yeast with splicing defects (JBe), and analysis of splicing in plants (JBr,ABa, AJ). This collection of technologies offered by the network will be the basis for direct one on one contactsbetween participating labs to set up a highly connected network of various kinds of cooperation on single researchprojects or shared uses of facilities and equipment.2. Establishment of an online knowledge database (controlled access) containing uniformly formatted protocols anduseful information such as a list of sequences used for successful down-regulation of target genes by RNAi (GAk,SS, RL) and proteomics data (RL, AL). This database will be curated to ensure that reviewed information enteringthe database is always kept up to date. User feedback and a list of contact persons for each deposited protocol will beincorporated into the database structure.3. Sharing of facilities set up by participants, such as datasets and analysis programs (GAs).4. Identification and network dissemination of the availability of mouse monoclonal antibodies and rabbit antisera asthey become available (RL, SS).These arrangements will be followed during the remainder of the funding period by more widespread sharing ofexpertise, which will usually involve movement of young trainee scientists from one laboratory to another (usuallyin different countries). In addition, some laboratories run intensive workshops, and these can be opened up to postdocsand students from the network (e.g., the FISH and immunocytochemistry workshop run annually in Jerusalem:HS).Deliverables263 Manuscript of a book working title : “ Methods in alternative pre-mRNA processing” (month 48) (continuationof 153)253