PDF file: EURASNET Annual Report 2008

J. Beggs has had frequent contact with Reinhard Rauhut regarding EURASNET business, especiallyabout issues relating to reporting and meeting organization.b) Major cost items with justificationParticipant 1A Type of expenditure Budget total costspersonnel 23.484,38consumables 10.843,99training 0equipment 0travel 20,81= total direct costs+ overhead 6.869,83= total eligible costs 41.219,01Major cost items• ResearchPersonnelEuroEuroO. Cordin [WP 9] 553 hours 11.563,02R. Grainger [WP 9] 402 hours 11.921,36ConsumablesOligonucleotidesChemicalsEnzymesAntibodiesTotal:0.7K Euro5.4K Euro2.7K Euro2.0K Euro10.800 EuroDNA oligonucleotides are used as probes and primers for microarray analyses and to performquantitative (Real Time) PCR to assess RNApol II and splicing factor recruitment in prp45 mutantstrains.Chemicals includes Cy-dyes for microarrays, Protein A Sepharose and IgG agarose forimmunoprecipitations, and culture media necessary to grow yeast and bacterial cell culturesEnzymes such as reverse transcriptase, Taq polymerase and specific RNA inhibitors are necessary toperform PCR analysis.Commercial antibodies were purchased for ChIP analyses and secondary antibodies for westernblotting.Travel:21 EuroTravel insurance for Richard Grainger and Vanessa Cristao to travel to the EURASNET meeting inKrakow• DisseminationTravel for J. Beggs and R Grainger to the Annual Meeting in Krakow, May, 2008.Two members of my research group attended the intranet meeting at the RNA UK Workshop,and another attended the Career Development Workshop in Krakow.But these are not claimed here.149