Sažeci 2. Hrvatskog kongresa medicinske biokemije - Klinički zavod ...

POSTERS
07-6/P4
NEUTRAL GLYCOSPHINGOLIPIDS AND GANGLIO-
SIDES IN SKELETAL MUSCLE OF A2G-ADR MOUSE
M. Cačić , I. Kračun , J. Muthing
Croatian Institute for Brain Research, School of Medicine, Zagreb, Croatia
2 Technical Faculty, Bielefeld, Germany
The expression of neutral glycosphingolipids (GSLs) and gangliosides
was investigated in crvosections of normal mouse skeletal muscle
and in muscle of A2G-adr mouse mutant (a model for human recessive
myotonia of Becker type) using indirect immunofluorescence microscopy.
Transversal and longitudinal sections were immunostained
with specific polyclonal and monoclonal antibodies against different
GSLs (lactosylceramide, gangliotetraosylceramide, lacto-N-neotetraosylceramide,
globoside, GM3(Neu5Ac), GM3(Neu5Gc) and GMl(Neu
5Ac)). In normal CBA/J mouse muscle the main immunohistochemically
detected ganglioside was GM3(Neu5Ac) followed by moderately
expressed GM3(Neu5Gc) and GM1. Neutrally stained GSLs were
lactosylceramide and globoside with almost identical high fluorescence
intensity Low quantities of Forssman GSL were immunostained. No
gangliotetraosylceramide was detected. Ali GSLs were detected in
sarcolemma, but also in considerable amounts on intracellular level.
Different expression of GSLs was found in muscles of A2G-adr mouse
mutant, not so in the quantities of the compounds, but in their distribution
along the membranes. The evidence of translocation of GSLs
in A2G-adr muscle sarcolemma are reported for the first time in this
disease, however, without explanation.
07-8/P1
LAKTAT U PREDVIĐANJU KARDIOGENOG ŠOKA
Š. Dvornik, J. Mihić, K. Draženović
KBC Rijeka
Kardiogeni šok je glavni uzrok smrti bolesnika s akutnim infarktom
miokarda (AIM). Prvi klinički znak kardiogenog šoka kod AIM
predstavlja slaba periferna perfuzija. Prema Shepsu i Kessleru odre-
BIOCHEMIA MEDICA god. 6, br. 1, 1996 155