Sažeci 2. Hrvatskog kongresa medicinske biokemije - Klinički zavod ...

07-14/P11
THE EFFECTS OF THE SERA OF INJURED PATIENTS
ON THE GROWTH OF THE HUMAN PERIPHERAL
BLOOD MONONUCLEAR CELLS: POSSIBLE
INVOLVEMENT OF THE HEPARIN BINDING
HUMORAL FACTORS IN THE PHENOMENON
OF ENHANCED OSTEOGENESIS IN THE PATIENTS
WITH TRAUMATIC BRAIN INJURY
R. midburger 2 , S. Borović 1 , N. Žarković 1 , A. Meinitzer 2 , W. Petek 2 , M.
Jurin
Ruđer Bošković Institute, Zagreb; University Clinic of Traumatologa Graz,
Austria
Among the most provocative phenomena is the phenomenon of
enhanced osteogenesis in patients with traumatic brain injury (TBI).
In these patients very early consolidation of bone fractures is followed
by hypertrophic callus formation or with heterotopic ossifications.
Although the mechanism of this phenomenon is not clarified, it seems
to be based on certain, not yet identified, humoral factors influencing
tissue regeneration. Thus, the aim of the study was to get some information
about the possible influence of such humoral factors on the
immune system and, hence, indication on their nature and the involvement
of the immune system in the phenomenon itself. We have
analysed the growth of the normal human peripheral blood mononuclear
cells (PBM) incubated in the presence of sera of differently
injured patients and the same sera after heparin-affinity chromatography.
Four experimental groups comprised subjects with the following:
1) fractures (FRA) of long bones or large joints (n = 9), 2)
traumatic brain injury (TBI) only (n = 11), 3) combined traumatic
brain injury and the bone fractures (TBI + FRA) (n = 10), and also 4)
healthy volunteers. Effects of the sera prepared as pooled samples
obtained weekly during the first four posttraumatic weeks on the
growth of the PBM in the presence of 2,5 g/ml PHA-L or in the absence
of the mitogen, determined by the intensity of the H-thymidine
incorporation, were compared with the effects of the same concentration
(1%) of the normal human pooled (n = 10) serum. In ali
samples of injured patients sera, decreased PHA reactivity was observed
with the tendency of recovery in the fourth week after injury
except in TBI + FRA group of patients where there was no recovery
during the whole period. Removal of heparin binding humoral factors
from the sera caused changes in PBM reactivity on mitogen. In ali
BIOCHEMIA MEDICA god. 6, br. 1, 1996 179