Sažeci 2. Hrvatskog kongresa medicinske biokemije - Klinički zavod ...

POSTERSKI RADOVI
Sažeci 2. Hrvatskog kongresa medicinske biokemije
ženi mogućem trovanju olovom (n = 33). Katalitička koncentracija NAG
i koncentracija EP izmjerene su spektrofluorometrijskim metodama,
D-DALK standardiziranom metodom koja se primjenjuje u Europi i
olovo metodom elektrotermičke atomske apsorpcijske spektrometrije.
Rezultati su pokazali da se katalitičke koncentracije NAG u serumu
i mokraći ne razlikuju značajno od vrijednosti u kontrolnoj skupini.
Rezultati PbK, D-DALK i EP u 33 ispitivana djelatnika pokazuju da
je razina njihove apsorpcije olova unutar granica za našu opću populaciju,
a pogotovo unutar dopustivih bioloških graničnih vrijednosti za
profesionalnu izloženost olovu (PbK 400 (ig/L, D-DALK 151 J/L Ere,
EP 2,67 iimol/L Ere). Preporuča se da se u djelatnika koji su profesionalno
izloženi olovu nakon određenog vremena kontrolira koncentracija
primijenjenih pokazatelja toksičnog djelovanja olova i na taj način
spriječi razvoj nepovratnih promjena u organizmu.
07-12/P9
SOME CHARACTERISTICS OF SOMATOSENSORY
EVOKED POTENTIALS IN 23 DAYS METHADONE
TREATED RATS
M. Bjegović 1 , M. Slijepčević , S. Sakoman , V. Išgum
1 Ruđer Bošković Institute, 2 CH "Sisters of Mercy", 3 CHC Zagreb; Zagreb
Methadone is a svnthetic analgetic and a derivate of heptanone.
It serves as substitute drug for heroine abusers. Methadone has analgetic,
sedative and antitussive action and exerts hvpnotic and euphoric
action as well. The mechanism of the drug action is still poorlv
understood, especiallv in diabetic individuals. Hyperglycaemia is a
causative factor for various neuropathies. We studied somatosensorv
evoked potentials (SEPs), simultaneouslv recorded in healthv and diabetic
Wistar rats treated 23 days with methadone (0,87 mg/kg) per os
daily. Diabetes was induced by alloxan monohydrate (75 mg/kg b.w.i.v).
Our computerized method with adapted struetures of hardware and
softvvare, necessary for recoding of SEPs in small animals, was used.
SEPs analysis showed an enormous inerease pf P13 amplitude; furthermore,
peak-to-peak amplitude P13-N20 was augmented (148%,
p/0,05) in diabetic methadone treated rats. On the other hand, N29
amplitude was decreased (22,2%; p/0,01). The latencies of P13, P25
and P31 were significanth/ (p/0,01) prolonged (P13 to 113,9%; P25 to
110,1% and P31 to 111,9%). The same waves were affected in the
BIOCHEMIA MEDICA god. 6, br. 1, 1996 167