SLEEP 2011 Abstract Supplement

A. Basic Science VI. Chronobiology
photic responses in Per2-deficient (Per2 KO) and albino wild-type (WT)
mice.
Methods: General locomotor activity was measured using infrared sensors
interfaced with a ClockLab data acquisition system. Phase-shifts
were measured using an Aschoff Type II procedure. In Experiment 1,
WT and Per2 KO mice received a cocaine (20 mg/kg) or saline injection
at midday. In Experiment 2, WT and Per2 KO mice received the cocaine
or saline injection 15 min before the presentation of phase-delaying light
pulse in the early night. In Experiment 3, WT mice were outfitted with
a microdialysis probe targeting the SCN. Cocaine dissolved in ACSF or
ACSF alone was perfused for 80 min starting at midday.
Results: In Experiment 1, cocaine advanced rhythms relative to saline
in both genotypes (p