Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

Removing sequences from a contig
To remove sequences from a contig, click on the name of the sequence you wish to
remove from the list at the left side of the Contig Editor window or in the Overview
window. To remove more than one sequence at a time, hold down the Shift key while
you click the names. Next, go to the Contig menu and choose Remove Selected
Sequences… Sequencher will ask you if you are sure you wish to remove the
sequence(s). Click on the Remove Fragments button to delete the sequences from the
contig.
The sequences you just removed from the contig are moved out of the contig and back
into the Project Window. They will also stay selected.
Note: This action may break a contig into multiple pieces. If this is about to happen,
Sequencher will display a dialog box (Figure 12-7) to ask if this is really what you intend
to do. Press the Cancel button if you do not wish to proceed. Any new contigs generated
in this way are named by appending an alphabetic character to the name of the original
contig.
Figure 12-7 Warning dialog when about to make a hole in a contig
Dissolving a contig
To dissolve a contig, first select the contig’s icon from the project. Go to the Contig menu
and choose Dissolve Contig…. You can also perform this command while you are in the
contig editor.
Sequences from the dissolved contig are shown highlighted in the project. It is important
to remember that all edits made to a sequence while in the contig will be retained. If you
want to restore the original sequences you must go to the Sequence menu and click on
Revert to Experimental Data.
SEQUENCHER 4.8 User Manual for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.
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