Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

Starting from the Project Window, go to the Select menu and click on the Select All
command to select all the sequences in your project. Click on the Auto Assemble by
Name button, or the To Reference by Name button if you have a Reference Sequence.
The Assembly Preview window will appear. This window contains the names of the
contigs that may be generated using your chosen handle and the probable number of
fragments in each contig. Click on the Assemble button to continue with the assembly,
or click on the Cancel button.
Sequencher displays the Assembly Completed dialog window when the assembly has
finished assembling your sequences.
Figure 10-7 The Assembly Completed window
This dialog window gives a summary of the assembly process. If this information is
sufficient, click the Close button. This will dismiss the Assembly Completed window.
If you require more detailed information, click on the Details… button. You will see a
report that contains further information about the assembly. You can save a text copy of
this report by clicking on the Save As button. Dismiss the dialog window by clicking the
Close button.
Note: The name of each contig formed using Assemble by Name will be based on the
Assembly Handle used.
SEQUENCHER 4.8 User Manual for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.
79