Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

menu and choose Import then the subcommand Sequence From VecBase. The Vector
Selection dialog box appears; choose the desired file and click Select. If you wish
to import more than one file you can use Shift or Command click for continuous or
discontinuous listings of sequences.
Creating a new sequence
Another way to bring new data into Sequencher is to create a new sequence fragment.
To do this, go to the Sequence menu and choose Create New Sequence…
Sequencher displays a dialog box asking you to name the new sequence (Figure 4-3).
The default name, in this case “Frag[0001],” can be set as a user preference. A sequence
name can be a maximum of 255 characters, including any punctuation and spaces, but 31
characters is recommended for export functions.
When you have typed the name you want (or if you accept the default name), click OK
or press the Enter key. A new Sequence Editor appears showing the name you selected in
the title bar. The new Sequence Editor lets you enter and change sequence data. Just type
as you would with a standard word processor.
When you are finished entering and/or editing a sequence, click the close button in the
top left corner of the window at the left end of the title bar or go to the File menu and
choose Close Window. Sequencher asks if you have finished editing. At this point
you can record your data as experimental data or you can tell Sequencher you have
not yet finished editing by selecting the appropriate button. Sequencher then removes
the Sequence Editor window from the screen. The sequence is now represented by a
sequencing icon in the Project Window.
Figure 4-3 Create New Sequence dialog box
Double-clicking a project icon
When you double-click on a sequence project icon, this launches Sequencher and opens
the project. If a Project Window is already open, when you double-click on a project icon
Sequencher will ask whether you want to save changes to your current project before
closing it. Click the Cancel button if you do not want to proceed. If you want to close the
project and save any changes you have made, click the Yes button. If you want close the
project without saving your changes, click the No button.
SEQUENCHER 4.8 User Manual for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.
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