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Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

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5. Organizing Project Views<br />

In this chapter on organizing project views you will learn more about the structure of a<br />

<strong>Sequencher</strong> project as you begin to work with your data. We will discuss how to select<br />

individual <strong>and</strong> multiple sequences using various techniques, renaming items <strong>and</strong> saving<br />

your work.<br />

Working with icons <strong>and</strong> lists<br />

Changing between project views<br />

<strong>Sequencher</strong> provides you with a variety of ways to view <strong>and</strong> organize the contigs <strong>and</strong><br />

unincorporated data in your Project Window. You can view your data as large or small<br />

icons or in list form (for more information see Chapter 3 “The Project Window”). You<br />

can toggle quickly between types of views by holding down the Alt key <strong>and</strong> clicking in<br />

the header title area, just above the icons <strong>and</strong> below the parameter information. You can<br />

display a list of sequences in a single contig in the list view by clicking on the plus sign<br />

to left of the contig icon. You can see a list of sequences in all your contigs by going to<br />

the View menu <strong>and</strong> choosing Exp<strong>and</strong> All.<br />

Collecting sequences in refrigerators<br />

<strong>Sequencher</strong> lets you create “refrigerators” in the Project Window to hold certain subsets<br />

of your project. Refrigerators hold only raw sequences, not contigs or other refrigerators.<br />

To “put away” a few sequences in “cold storage,” select the sequences <strong>and</strong> then go to the<br />

Edit menu <strong>and</strong> choose Refrigerate. <strong>Sequencher</strong> collects the sequences <strong>and</strong> puts them in<br />

the refrigerator.<br />

To remove sequences from the refrigerator, open the refrigerator by double-clicking on it.<br />

You will see a list of items. Select the items you want to remove <strong>and</strong> then click the button<br />

called Move Selected Items To Project Window to execute the comm<strong>and</strong>.<br />

SEQUENCHER <strong>4.8</strong> <strong>User</strong> <strong>Manual</strong> for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.<br />

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