Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

Once you have made your selection click the OK button. The Variance Table will be
rebuilt automatically.
Figure 13-8 Comparison Range dialog window
Restricting the Translation Range
Reference Sequences are often marked up with GenBank-style features. (See Chapter 17
“Motifs and Features” for further information).
You can reduce the scope of your Translated Variance Table to a single feature. Some
features such as mRNA or CDS may be composed of several elements. These elements
are concatenated and treated as a single feature. Translation of the DNA sequence starts
in the first frame, with the first base in the feature range. Figure 13-9 illustrates an
unfiltered Comparison Range, a joined feature (blue) and a single feature (pink).
Figure 13-9 Restricting the Comparison Range using Features
View your Translated Variance Table and go to the button bar and click on the
Translation Range button. The Translation Range dialog window appears. You will see
that the Translate Entire Sequence radio button is checked. This is your default. Leave
this radio button checked if you decide to leave your table unfiltered.
SEQUENCHER 4.8 User Manual for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.
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