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Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

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Name<br />

Project<br />

Project window<br />

Quality score<br />

Radio button<br />

Reference<br />

sequence<br />

Refrigerator<br />

SCF<br />

Secondary<br />

peak<br />

Sequence<br />

Sequence Editor<br />

Slider<br />

Title bar<br />

VecBase<br />

Definition<br />

A project contains sequences, information on how these sequences fit<br />

together to form larger pieces <strong>and</strong> parameter information, specified by<br />

the user, that controls alignment operations.<br />

The window in which <strong>Sequencher</strong> displays the contigs <strong>and</strong><br />

unincorporated Sequences of a project. Items in the Project Window<br />

are can be shown as icons, small icons or as a sorted list.<br />

A number associated with each base call <strong>and</strong> which defines the<br />

likelihood that a base call is incorrect. See also Confidence score.<br />

A control that lets you choose among alternate possibilities. Radio<br />

buttons always appear in sets of at least two. Click one of the buttons<br />

to select it. A button that is selected is shown with a filled circle;<br />

buttons that are not selected are shown with open circles.<br />

A sequence used as a prototype or benchmark sequences. If a sequence<br />

has been designated as Reference within <strong>Sequencher</strong> it has special<br />

properties.<br />

A special folder in which to store subsets of sequences which need to<br />

remain in your project.<br />

St<strong>and</strong>ard Chromatogram Format. A format used to store analyzed data<br />

or fragment data for a single sample.<br />

A chromatogram peak whose height may be less than that of the<br />

primary peak <strong>and</strong> whose presence may indicate that a heterozygote<br />

exists at that position.<br />

A small piece of DNA, decoded into its component bases, in order,<br />

represented as text. Sometimes in reverse order, depending upon the<br />

vagaries of the sequencing procedure.<br />

The window used for editing DNA sequences. This editor looks<br />

much like a word processor <strong>and</strong> supports the st<strong>and</strong>ard text editing<br />

comm<strong>and</strong>s: Cut, Copy, Paste <strong>and</strong> Undo.<br />

An on screen control that simulates the action of a slide control that<br />

might normally be used as a volume or dimmer control on a household<br />

appliance.<br />

The region at the top of a window on the computer.<br />

A cloning vector sequence database prepared by Friedhelm Pfeiffer,<br />

in collaboration with William Gilbert. Also a file with this name, in<br />

VecBase (CODATA) format, Part of the <strong>Sequencher</strong> distribution.<br />

SEQUENCHER <strong>4.8</strong> <strong>User</strong> <strong>Manual</strong> for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.<br />

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