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Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

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Format: drop down menu allows you to choose the format type for your exported<br />

sequence. Clicking on the Options… button displays the window where you turn options<br />

on or off. In most cases you will want to just click on Export <strong>and</strong> use the original<br />

consensus name.<br />

The Browse… button opens a window called Browse For Folder. From this window<br />

you can choose where to store the new text file(s). You may create a new folder in which<br />

to store your sequences by clicking on the Make New Folder button at the bottom left of<br />

the window. Click on the OK button to record your chosen location.<br />

Uncheck the Use Default Names checkbox if you wish to set a specific export name.<br />

Exporting Contigs<br />

To export a contig, click on the item you want to export. Go to the File menu <strong>and</strong> click on<br />

Export <strong>and</strong> Contig… from the submenu.<br />

<strong>Sequencher</strong> displays the export dialog. The browse button allows you to choose a disk<br />

<strong>and</strong> folder to store the new text file(s). The format button allows you to choose the format<br />

type for your exported contig. You can choose from MSF, Nexus Interleaved, Nexus<br />

Sequential, Aligned FastA <strong>and</strong> CAF. You may also set a number of options. Clicking on<br />

the Options… button displays the window where you turn options on or off. Finally,<br />

clicking on the Export button executes the comm<strong>and</strong>.<br />

The Use Default Names option gives each export the name of its contig. Uncheck the<br />

Use Default Names checkbox if you wish to set a specific export name.<br />

The Browse… button opens a window called Browse For Folder. From this window<br />

you can choose where to store the new text file(s). You may create a new folder in which<br />

to store your sequences by clicking on the Make New Folder button at the bottom left of<br />

the window. Click on the OK button to record your chosen location.<br />

If you execute the Export comm<strong>and</strong> with the Sequence… suboption while a contig is<br />

selected, <strong>Sequencher</strong> will create a folder with the name of the contig. The folder contains<br />

all of the sequences in the contig.<br />

Exporting Selection as subproject<br />

If you wish to share your data with colleagues who may have older versions of<br />

<strong>Sequencher</strong> or another program, choose Export from the file menu <strong>and</strong> then Selection<br />

As Subproject…, then select the appropriate version or type from the Format dropdown<br />

menu.<br />

In certain cases, you may want to move some but not all items from one <strong>Sequencher</strong><br />

project into another. Working in the Project Window, select the sequence fragments <strong>and</strong><br />

contigs you want to export. Go to the File menu <strong>and</strong> choose Export <strong>and</strong> then choose<br />

Selection As Subproject. <strong>Sequencher</strong> creates a new project containing only the<br />

items you selected. Now you can import the newly created sub-project into the chosen<br />

<strong>Sequencher</strong> project.<br />

SEQUENCHER <strong>4.8</strong> <strong>User</strong> <strong>Manual</strong> for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.<br />

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