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Sequencher 4.8 User Manual--PC - Bioinformatics and Biological ...

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Variance Table<br />

The Variance Table preferences control how ambiguous matches <strong>and</strong> large gaps will<br />

be treated. If you wish to include ambiguous matches in your table, click the Include<br />

Ambiguous Matches checkbox. If you wish to exclude large gaps from your table, click<br />

the Exclude Large Gaps checkbox.<br />

When you generate a Variance Table you would expect to have blank cells where the<br />

base at that position matches the exemplar. If you would like to see all bases across the<br />

comparison range whether they match or are different to the exemplar then click the<br />

Populate All Cells checkbox (see Figure 21-18).<br />

Figure 21-18 Variance Table preference settings<br />

Input/Output<br />

File Import<br />

File Import preferences control how <strong>Sequencher</strong> applies the automatic trim function to<br />

imported files.<br />

Use the checkboxes grouped under Add Imported Sequences To Trim Window to<br />

specify which sequences <strong>Sequencher</strong> should examine for poor quality ends <strong>and</strong> then trim.<br />

You can also specify how you want to resolve conflicts between the names of imported<br />

files <strong>and</strong> names stored with particular sequences. If you click the Prefer File Names To<br />

Data Names checkbox, <strong>Sequencher</strong> uses the file name.<br />

Clicking the Review Trim Parameters button displays the Ends Trimming dialog box,<br />

in which you can set trim criteria. (See Chapter 6, “Preparing your Data for Assembly,”<br />

for details.)<br />

SEQUENCHER <strong>4.8</strong> <strong>User</strong> <strong>Manual</strong> for Windows © 1991 - 2007 Gene Codes Corporation, Inc. All rights reserved.<br />

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